【摘要】 目的检测ki-67在正常结直肠组织和结直肠癌中的表达情况,探讨其表达与结直肠癌Dukes分期关系,为临床结直肠癌的诊疗判断提供参考。方法收集临床病理资料完整的结直肠癌54例,正常结直肠黏膜组织28例。应用免疫组化法(SP法)检测正常结直肠组织和结直肠癌中ki-67蛋白的表达。结果Ki-67蛋白在结直肠癌及正常结直肠黏膜中的表达率分别为85.19%和10.71%,在结直肠癌组织中的表达高于正常结直肠黏膜的表达,差异有统计学意义(P<0.01)。ki-67蛋白的表达和Dukes分期无相关性,差异无统计学意义(P>0.05)。结论ki-67蛋白过表达与结直肠癌的发生发展密切相关,但不能作为结直肠癌预后的参考指标。更多还原

【Abstract】 Objective To study the expression of ki-67 in colorectal carcinoma and normal mucous of large intestine,explore its correlation with Dukes’staging and provide clues to the diagnosis and therapy of colorectal cancer. Methods 54 specimens of colorectal carcinoma,28 normal mucous of large intestine and their corresponding intertity clinic-pathological factors were obtained. The expression of ki-67 was detected by immunohistochemical staining S-P method. Results Positive expression of ki-67protein by immunohistochemistry in colorectal carcinoma tissues and normal mucosa of large intestine was 85.19% and 10.71% respectively. The expression of ki-67 protein was significant higher in colorectal carcinoma tissues than that in normal mucous of large intestine (P<0.01). No significant correlation was found between the expression of ki-67 protein in colorectal carcinoma tissues and Dukes’staging of tumors(P>0.05). Conclusions Ki-67 protein had high correlation with the oncogenesis and development of colorectal cancer, but it shouldn’t be treated as a prognostic factor in colorectal cancer.更多还原