【摘要】 目的观察3-溴丙酮酸(3-BrPA)对SMMC-7721肝癌细胞增殖的抑制作用。方法对经0.25mmol/L～8.00mmol/L3-BrPA溶液处理的SMMC-7721细胞,采用MTT法检测细胞增殖抑制情况,并在荧光显微镜和电镜下观察细胞内部结构的变化。结果3-BrPA对SMMC-7721细胞的增殖有明显的抑制作用,其中在0.75mmol/L浓度下的抑制作用最强;经处理后的细胞,在荧光染色下显示坏死细胞增多,在电镜下显示细胞染色质分散,少量核染色质凝聚。结论3-BrPA对SMMC-7721肝癌细胞有抑制作用。更多还原

【Abstract】 Objective To observe the inhibitory effect of 3-bromopyruvate on the cell proliferation of SMMC-7721 human liver cancer cell line. Methods The SMMC-7721 cells were treated with different concentrations of 3-bromopyruvate,then the cell growth was assessed by MTT and the apoptotic or necrotic cell death was examined by fluorescent staining and by electron microscopy. Results The 3-bromopyruvate at dose of 0.75mmol/L inbibited SMMC-7721 cell proliferation;The necrotic cells were observed by fluorescent staining and the apoptotic cells observed under electron microscope. Conclusion 3-bromopyruvate could inhibit the proliferation of SMMMC-7721 cells.更多还原