【摘要】 目的观察体外P38MAPK信号通路抑制剂SB203580单独及SB203580联合mTOR(哺乳动物雷帕霉素靶蛋白)信号通路抑制剂雷帕霉素(Rapamycin)对Ishikawa细胞生长、细胞凋亡以及细胞侵袭能力的作用。方法一定浓度梯度的SB203580单独或联合运用雷帕霉素,MTT(四甲基偶氮唑蓝)法检测细胞抑制率、软琼脂克隆形成实验检测肿瘤细胞体外成瘤性、流式细胞仪检测细胞凋亡、Transwell法检测Ishikawa细胞体外侵袭力的改变。结果SB203580对Ishikawa细胞有抑制生长、降低体外成瘤性、诱导凋亡和降低细胞侵袭能力的作用;在相同条件下,SB203580与雷帕霉素联合的抗肿瘤作用要明显优于SB203580单用。结论SB203580与雷帕霉素可协同抑制人子宫内膜癌细胞Ishikawa的生长、诱导细胞凋亡,并同时抑制Ishikawa细胞的侵袭性,具有良好抗肿瘤前景。更多还原

【Abstract】 Objective To investigate the synergistic effect of SB203580 and rapamycin on cell invasion and apoptosis of human endometrial carcinoma Ishikawa cells in vitro.Methods Different concentrations of SB203580 alone or in combination with rapamycin were given to cultured Ishikawa cells in vitro.Cell viability was determined using MTT method.Clone formation test was used to detect oncogenicity in vitro.double staining with Annexin-V-FITC was used to detect cell apoptosis by the flow cytometry(FCM).Cell invasive ability was measured with Transwell.Results SB203580 inhibited cell viability and oncogenicity,induced cell apoptosis and decreased invasive ability of Ishikawa cell.The anti-tumor effect of SB203580 in combination with rapamycin was better than that of SB203580.Conclusion SB203580 and rapamycin synergistically inhibit proliferation and invasive ability of Ishikawa cell and induce cell apoptosis.It is a promising anti-tumor agent.更多还原