【摘要】 荧光定量PCR技术不仅实现了PCR从定性到定量的飞跃,更以其特异性强、灵敏度高、重复性好、定量准确、速度快、全封闭反应等优点而成为分子生物学研究的重要工具,并用于生物特异核苷酸的检测。主要概述了荧光染料法、TaqMan探针法、分子信标法、Lux Primer 4种荧光定量技术的原理、特点以及荧光定量PCR技术在各方面的应用。更多还原

【Abstract】 Fluorescence Quantitative Polymerase Chain Reaction(FQ-PCR,Real-time PCR or kinetic PCR) became an essential method in molecular biology study and the detection of biological special nucleotide not only because it is quantitative except qualitative but also because its characteristics such as high specificity,high sensitivity,replicable,precise quantification,rapid,and totally closed reaction.This paper reviewed the principles,characteristics,and their application in certain aspects of the 4 fluorescence quantitative PCR techniques such as fluorescence dye,TaqMan fluorescence probe,molecular beacon PCR,and Lux Primer.更多还原