【摘要】 对根霉脂肪酶储存稳定性(干燥状态)及在水中,甘油微量水中等不同体系的热失活机理进行了研究,并求出了动力学参数.结果表明该酶在不同的外部环境中热失活机理并不一致:干燥酶粉符合一级失活模型,酶水溶液和酶甘油微水溶液却符合两步串联失活机理,而且证实甘油对该脂肪酶的热稳定性有促进作用.更多还原

【Abstract】 Thermal inactivation behaviors of the Rhizopus sp.Lipase in several systems were investigated.Thermal inactivation kinetics depend on the environment around the lipase: the dry powder form of the lipase follows a simple One-step two states while both the lipase in water and the lipase in glycerol containing minor amount of water(4 wt.%) properly fit Two-step series deactivation.Glycerol was confirmed to be in favor of the stabilization of the lipase.Furthermore,kinetics parameters of the inactivation models were calculated by statistically linear or non-linear regression.更多还原