【摘要】 目的研究死后大鼠肌肉组织中微生物ATP浓度变化及其与死亡时间(postmortem interval,PMI)的关系。方法取健康SD大鼠处死,死后不同时间取肌肉组织,用生物发光法检测微生物ATP浓度,对所得数据进行统计学处理。结果死后肌肉组织中微生物ATP含量随时间延长而增加,于7d达到高峰,随后逐渐降低,并于10d再次略微升高。PMI在0～9d内与肌肉中微生物ATP含量变化拟合最优。以PMI为自变量的三次多项式回归方程为:y=0.020x3-0.166x2-0.666x+13.412(r2=0.989,P<0.01)。结论死后肌肉组织中微生物ATP含量变化可用于PMI推测;本检测方法所需检材量小,不受组织自身降解的影响。更多还原

【Abstract】 Objective To study the relationship between the changes of microbial ATP in rat muscle tissue and the postmortem interval(PMI). Methods Healthy SD rats were sacrificed and their muscles were sampled at different postmortem intervals. The concentration of microbial ATP was detected using bioluminescent assay and the data was statistically analyzed. Results The content of microbial ATP in rat muscle tissue increased along with PMI extension and peaked on postmortem day 7, thereafter decreased gradually, but increased slight- ly on postmortem day 10 once again. The PMI correlated best with the content of microbial ATP in rat muscle tissue within 9 days. If the PMI was the independent variable, the cubic polynomial regression equa- tion was y=0.020 x3-0.166 x2-0.666 x+13.412(r2=0.989, P<0.01). Conclusion Changes of microbial ATP content in rat muscle tissue may be used for estimation of PMI. Since this assay requires only small amount of tissue with much less influence by self-decomposition, it may broaden the time range of PMI estimation.更多还原