节点文献

川芎嗪对糖基化终末产物诱导肾小管上皮细胞外基质表达的影响

Effects of Ligustrazine on Expression of Extracellular Matrix in Human Proximal Renal Tubular Epithelial Cells Induced by Advanced Glycation End Products

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 许艳芳林久茂江德文李桂芬

【Author】 XU Yan-fang1,LIN Jiu-mao2,JIANG De-wen1,et al(1. The First Affiliated Hospital,Fujian Medical University,Fuzhou,Fujian 350005,China;2. Fujian Academy of Integrative Medicine,FUTCM,Fuzhou,Fujian 350108,China)

【机构】 福建医科大学附属第一医院福建中医学院中西医结合研究院

【摘要】 目的观察川芎嗪对AGE-BSA诱导人近端肾小管上皮细胞(HK-2)细胞外基质(ECM)成分表达的影响,探讨其干预糖尿病肾间质纤维化的作用机制。方法将HK-2细胞在体外与BSA、AGE-BSA、AGE-BSA+川芎嗪(不同浓度)共培养72h后,用RT-PCR和Western Blot分别检测FN、ColⅠα1mRNA及蛋白的表达。结果RT-PCR和Western Blot结果显示,100μg/ml BSA刺激HK-2细胞后,FN、ColⅠα1mRNA及蛋白表达无明显增加;100μg/ml AGE-BSA刺激后,FN、ColⅠα1mRNA及蛋白表达明显增加,与空白对照组及相同浓度BSA组相比,P<0.01;加入(40~120)μg/ml川芎嗪共同干预后,川芎嗪可在mRNA及蛋白水平呈剂量依赖性下调AGE-BSA诱导的FN、ColⅠα1的表达。结论川芎嗪能抑制AGE-BSA诱导的HK-2细胞FN、ColⅠα1的合成。表明川芎嗪能通过抑制肾小管上皮细胞分泌ECM进而延缓糖尿病肾间质纤维化的进展。

【Abstract】 Objective To investigate the effect of ligustrazine on the expression of extracellular matrix(ECM) components in human renal proximal tubular cells(HK-2) induced by advanced glycation end products(AGEs) ,and explore it’s interventional mechanism on tubulointerstitial fibrosis in diabetic nephropathy. Methods HK-2 cells were cultured in vitro with bovine serum albumin(BSA) ,AGE-BSA and recombinant AGE-BSA and ligustrazine respectively for 72 h. The expressions of FN and ColⅠα1 mRNA and protein were detected by RT-PCR and western blot respectively. Results The expression levels of FN and Col Ⅰα1 mRNA and protein were up-regulated induced by 100 μg/ml AGE-BSA compared with the blank control group and the same concentration group of BSA(P<0.01) . 40~120 μg/ml ligustrazine was added to the culture solution,the expression levels induced by AGE-BSA were dramatically down-regulated in a dose-dependent manner. Conclusion Ligustrazine negatively regulates the synthesis of FN and ColⅠα1 induced by AGE-BSA. This shows that ligustrazine can delay the development of tubulointerstitial fibrosis in diabetic nephropathy by inhibiting the accumulation of ECM in HK-2.

【基金】 福建省卫生厅青年科研课题基金资助项目(2008-1-29);福建省高校中西医结合基础重点实验室开放课题基金(ZXY2008002);2008年度陈可冀中西医结合发展基金项目(CKJ2008006)
  • 【文献出处】 福建中医学院学报 ,Journal of Fujian University of Traditional Chinese Medicine , 编辑部邮箱 ,2009年01期
  • 【分类号】R285.5
  • 【被引频次】5
  • 【下载频次】116
节点文献中: 

本文链接的文献网络图示:

本文的引文网络