【摘要】 研究了纳米羟基磷灰石(nano-HAP)颗粒静脉注射后在小鼠体内的分布、蓄积和排泄情况。将中子活化的nano-HAP尾静脉注射到小鼠体内,剂量为9mg/kg。分别在15min、1h、2h、4h、8h、24h、72h时断髓处死动物,解剖并收集动物的血液、脑、心、肝、脾、肺、肾、胃、小肠、胸骨、子宫或睾丸、粪便和尿液。用高氯酸和双氧水将收集的样品消解后,通过液体闪烁计数器测定各脏器组织和排泄物中的45Caβ射线计数来反映nano-HAP在动物体内的分布。结果显示,尾静脉注射nano-HAP后,大部分组织中45Caβ射线计数范围为0.5～250Counts/min·mg,肺、肝、脾这3个部位中的45Caβ射线计数相当于其它脏器组织的6倍以上。整个试验期间,只有骨骼中的45Caβ射线计数变化不大,其它组织中的45Caβ射线计数均随时间延长而减少。各个时间点粪便中45Caβ射线计数要明显大于尿液中45Caβ射线计数。nano-HAP颗粒可以在体内大部分的器官和组织中分布,其中肺、肝、脾是nano-HAP分布最多的几个器官,而骨骼是nano-HAP唯一的蓄积器官。粪便排泄是体内nano-HAP排泄的主要途径。更多还原

【Abstract】 The present study was designed to investigate the distribution, accumulation and excretion of hy-droxylapatite nanoparticles (nano-HAP) in mice with intravenous injection. The nano-HAP suspension was neutron activated and then stored 151d after neutron activation for experiment. The mice injected with radioactive nano-HAP heve sacrificed at 15min、1h、2h、4h、8h、24h、72h and the main organs were harvested for further measuring the counts of 45Ca in every sample by use of a liquid scintillation counter. Results indicate that 45Ca could be detected in all main organs (0.5~250Counts/min·mg), and there are more 45Ca exist in lung, liver and spleen. The counts of 45Ca in bone almost keep the same during the whole experiment period while counts of 45Ca of all other tissues decrease with time. And the counts of 45Ca exerted in faeces are higher than that in urine. So we can conclude that nano-HAP distribute in all organs of mice especially in lung, liver and spleen. But bone is the only tissue in which nano-HAP accumulated. Nano-HAP was excreted mostly through faeces.更多还原