【摘要】 为探讨硫酸镁(MgSO4)对电离辐射诱发脑损伤的保护作用,将成熟的SD大鼠36只随机分为空白对照组、实验对照组和硫酸镁实验用药组,用6MeV电子束对实验组大鼠进行20Gy全脑单次垂直照射,吸收剂量率为200cGy/min;实验用药组大鼠于照射前1d、照射后即刻和照射后连续5d分别给予10%的MgSO4腹腔注射,分别于照后第3、10、17和24d解剖大鼠,取其脑组织,用激光扫描共聚焦显微镜测定脑细胞内Ca2+的含量,硝酸还原酶法测定脑组织一氧化氮(NO)含量。结果显示,与空白对照组相比,实验对照组大鼠脑细胞内Ca2+平均荧光强度及脑组织中NO含量明显升高(P<0.05);与实验对照组相比,实验用药组大鼠脑细胞内Ca2+平均荧光强度及脑组织NO含量有所降低(P<0.05)。表明早期使用硫酸镁可抑制辐射引起的脑细胞内钙离子的超载及脑组织中NO含量的升高,硫酸镁对放射性脑损伤有保护作用。更多还原

【Abstract】 The work is to explore the protection of magnesium sulfate(MgSO4) on radiation-induced acute brain injury. Thirty six mature Sprague-Dawley(SD) rats were randomly divided into 3 groups of control, experimental control and experimental therapy group. The whole brains of SD rats of experimental control and experimental therapy group were irradiated with a dose of 20 Gy using 6 MeV electron beam. MgSO4 was injected into the abdomen of experimental therapy rats group 1day before, immediately and continue for 5days after irradiation respectively. The brain tissues were taken on 3, 10, 17 and 24 d after irradiation. Ca2+ content in brain cell was measured by laser scan- ning confocal microscopy, and the NO content in brain tissue was detected by the method of nitric acid reductase. Compared with the blank control group, the contents of Ca2+ in brain cell and NO in brain tissue of the experimental control group increase (P<0.05). Compared with the experimental control group, the two indexes of the experimental therapy group decrease (P<0.05). It shows that MgSO4 used in early stage can inhibit the contents of Ca2+ in brain cell and NO in brain tissue after radiation-induced acute brain injury. It means that MgSO4 has a protective effect on radiation-induced acute brain injury.更多还原