【摘要】 建立了用1,2-萘醌-4-磺酸钠测定阿魏酸哌嗪的新方法。在pH 13.0的KCl-NaOH缓冲溶液中,阿魏酸哌嗪与1,2-萘醌-4-磺酸钠反应形成红褐色产物,其最大吸收波长为484 nm。阿魏酸哌嗪浓度在0.4~80μg/mL范围内呈现良好线性关系。线性回归方程为A=0.07742+0.01216ρ(μg/mL),线性相关系数r=0.9986,表观摩尔吸光系数5.8×103L/mol/cm,相对标准偏差(RSD)和检出限分别为0.84%和0.29μg/mL。对药物样品中阿魏酸哌嗪的含量进行测定,平均回收率在95.1%~103.3%。更多还原

【Abstract】 A rapid,simple and sensitive method for the determination of piperazine ferulate using sodium 1,2-naphthoquine-4-sulfonate as a chromogenic reagent is established in this paper.It is based on the fact that a russety product can be formed by the reaction between piperazine ferulate and sodium 1,2-naphthoquine-4-sulfonate in pH 13.0 buffer solution.Beer′s law is obeyed in a range of 0.4～80 μg/mL for piperazine ferulate at the maximum absorption of 484 nm(ε=5.8×103 L·mol·cm-1).The linear regression equation of the calibration curve is A=0.07742+0.01216ρ(μg/mL),with a correlation coefficient of 0.9986.The detection limit is 0.29 μg/mL(3σ/k),RSD is 0.84% and recoveries are from 95.1% to 103.3%.Furthermore,the proposed method has been successfully applied to the determination of piperazine ferulate in pharmaceutical samples.更多还原