【摘要】 研究了次黄嘌呤猝灭伊文思蓝的荧光猝灭机理并根据次黄嘌呤对伊文思蓝荧光的猝灭作用建立了测定次黄嘌呤的新方法。线性回归方程为:ρ=6.292×103F-1-26.49,相关系数为0.9990,线性范围为0.20~20.0μg/mL,检出限为0.14μg/mL,相对标准偏差(RSD)为2.5%。求算了伊文思蓝与次黄嘌呤的形成常数K=2.63×102L/moL,实验了pH、放置时间、干扰离子等对测定的影响。更多还原

【Abstract】 The relative fluorescence intensity of evans blue(EB) decreased in the presence of hypoxanthine,which showed that fluorescence quenching occurred.A linear relationship was found between the relative fluorescence intensity of EB and the concentration of hypoxanthine.The linear regression equation of the calibration graph was ρ=6.292×103F-1-26.49 with a correlation coefficient of 0.9990 and the range of ρ was between 0.20 and 20.0 μg/mL.The detection limit was 0.14 μg/mL and the relative stand derivative was 2.5%.The binding constant K of 2.63×102 L/mol was obtained based on the fluorescence quenching results.Effects of pH,stability of EB in the presence of hypoxanthine,foreign ions on the determination of hypoxanthine have been examined.This method was simple,rapid and the detection limit was low.更多还原