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疫霉侵染的辣椒cDNA文库的构建及非特异性脂转移蛋白cDNA的筛选
Construction for cDNA library of pepper infected by Phytophthora capsici.,and the screening of nsLTP cDNA from library
【摘要】 建立了辣椒疫霉侵染的辣椒幼苗cDNA文库,该原始文库含有1.5×106个独立克隆,插入片段约为0.5-2 kb.采用基于PCR技术的96孔文库筛选方法,从该cDNA文库中筛选出了1个应答辣椒疫霉侵染的候选基因的全长cDNA.经生物信息学分析,推测这个候选基因为非特异性脂转移蛋白(non-specific lipid-transfer protein,nsLTP)基因.
【Abstract】 The total RNA was extracted from young pepper infected by Phytophthora capsici.,and the cDNA library including more than 1.5×106 individual clones was constructed with SMARTTM cDNA construction kit.A positive candidate resistant gene cDNA was isolated by 96-hole screening method based on PCR.The results of bioinformatics analysis showed that the candidate nsLTP resistant gene could code the non-specific lipid-transfer protein.
【关键词】 辣椒；
辣椒疫霉；
cDNA文库；
非特异性脂转移蛋白；
【Key words】 pepper； Phytophthora capsici.； cDNA library； non-specific lipid-transfer protein(nsLTP)；
【Key words】 pepper； Phytophthora capsici.； cDNA library； non-specific lipid-transfer protein(nsLTP)；
【基金】 国家自然科学基金(30571128、30600391);福建省自然科学基金(2008J0003)资助项目
- 【文献出处】 福建农林大学学报(自然科学版) ,Journal of Fujian Agriculture and Forestry University(Natural Science Edition) , 编辑部邮箱 ,2009年02期
- 【分类号】S436.418
- 【被引频次】7
- 【下载频次】171