【摘要】 目的检测巨噬细胞在肝细胞癌(HCC)中的分布规律及吞噬功能。方法收集14例HCC病人资料。利用流式细胞仪和免疫组织化学法(SP法)检测肝脏标本巨噬细胞(CD68)及其表面受体CD16的表达量,了解二者在肝组织中的位置及分布情况。结果癌组织中的CD68及其表面受体CD16表达量明显少于癌旁组织(均P<0.01)。巨噬细胞主要存在肝血窦处,呈卵圆、星形或纺锤形,胞浆呈棕黄染色;CD16着色于巨噬细胞胞浆,呈棕黄色,连续或灶性颗粒状分布。结论巨噬细胞及其表面受体表达量(CD16)在肝癌组织分布及表达少于癌旁组织,导致组织抗肿瘤能力低下。更多还原

【Abstract】 Objective To examine the distribution and phagocytic function of macrophages in hepatocellular carcinoma(HCC).Methods The data of 14 cases of HCC were collected.By using flow cytometry and immunohistochemistry,the expression,location and distribution of hepatic macrophages(CD68)and surface receptors CD16 in liver specimens were detected.Results Expression percents of macrophages(CD68)and CD16 in liver carcinoma tissues were significantly lower than that in the adjacent peritumoral tissues(all,P<0.01).Results Of immunohistochemical analysis showed that macrophages(CD68)mainly existed in hepatic sinusoid,appeared oval,stellate or spindly,and cytoplasmic was stained brown.CD16 were stained brown in the cytoplasm of macrophages,with a continuous or focal granular distribution.Conclusion Expressions and distributions of macrophage(CD68)and surface receptors CD16 in liver carcinoma tissues are less than that in the adjacent peritumoral tissues,which may be related to decreased resistance to tumor.更多还原