【摘要】 背景与目的PI3K/Akt/mTOR通路失调对肺癌形成可能具有重要作用,本研究通过分析非小细胞肺癌组织中PI3K/Akt/mTOR信号转导途径中VEGF、PI3K、Akt、mTOR基因的表达水平,探讨PI3K/Akt/mTOR信号转导途径与非小细胞肺癌之间的关系。方法外科手术中获取40例非小细胞肺癌组织及30例癌旁组织,应用逆转录聚合酶链反应(reverse transcriptase polymerase chain reaction,RT-PCR)技术检测癌及癌旁组织中VEGF、PI3K、Akt、mTOR基因的表达水平。结果非小细胞肺癌组织中VEGF、PI3K、Akt、mTOR基因的表达水平分别为(40±59)%、(61±23)%、(77±32)%、(43±21)%,癌旁组织中VEGF、PI3K、Akt、mTOR基因的表达水平分别为(16±40)%、(23±16)%、(10±12)%、(20±17)%,非小细胞肺癌组织中VEGF、PI3K、Akt、mTOR基因的表达水平均高于癌旁组织(P<0.01)。联合应用4个基因表达作为NSCLC诊断标志,诊断的敏感性达到87.5%,特异性为90%。结论非小细胞肺癌中PI3K/Akt/mTOR信号转导途径相关基因表达水平明显上调,说明该通路在非小细胞肺癌中被激活。联合检测VEGF、PI3K、Akt、mTOR的表达水平改变可能作为NSCLC的诊断标志。更多还原

【Abstract】 Background and objective It has been known that abnormality of PI3K/Akt/mTOR signal pathway plaied an important role in the oncogenesis of lung cancer.In this study,we tried to detect the mRNA expression levels of VEGF,PI3K,Akt,mTOR gene,the key genes of PI3K/Akt/mTOR signal pathway,in human non-small cell lung cancer(NSCLC) tissue and explore the relationship between PI3K/Akt/mTOR signaling pathway and non-small cell lung cancer.Methods Lung cancer tissue specimens were obtained from 40 patients.Adjacent-tumor NSCLC tissues from the 30 patients were served as control.The RT-PCR technique was used to detect the VEGF,PI3K,Akt,mTOR gene expression levels.Results The average mRNA expression levels of VEGF,PI3K,Akt,mTOR gene in lung cancer were(40±59)%,(61±23)%,(77±32)% and(43±21)% respectively,while the mRNA expression levels of VEGF,PI3K,Akt,mTOR genes in adjacent-tumor tissue were(16±40)%,(23±16)%,(10±12)% and(20±17)%,respectively.All the levels of VEGF,PI3K,Akt,mTOR gene expression in NSCLC were significantly higher than that in adjacent-tumor lung tissue(P<0.01).Conclusion Our preliminary data have demonstrated that PI3K/Akt/mTOR signaling pathway is activated in the tumor cells of NSCLC.The activated PI3K/Akt/mTOR signaling pathway might be an important role in the pathogenesis of NSCLC.更多还原