【摘要】 背景与目的肺癌转移相关基因-1(Pulmonary cancer Metastasis Associated Gene-1,MAG-1)是利用抑制消减杂交技术获得的与肺癌转移相关的基因。此研究的目的在于探讨基因MAG-1对肺巨细胞癌细胞的转移相关生物学行为的影响及其在24例肺癌组织中的表达情况。方法构建MAG-1的正反义表达载体,分别转染PLA801C和PLA801D,获得稳定表达相应序列的细胞株,观察稳定转染细胞的多种肿瘤转移相关能力的改变,并检测MAG-1对CD44、MMP-2等分子的影响,最后还检测了MAG-1在源于不同转移状况患者的肺癌标本中的表达情况。结果MAG-1对细胞生长、细胞与胞外基质的粘附能力以及侵袭能力均有促进作用;并能促进细胞中CD44和MMP-2的表达;MAG-1mRNA在有转移的肺癌中的阳性率(7/12)高于其在无转移的肺癌标本中的阳性率(2/12)。结论MAG-1具有增强肺癌细胞转移能力的功能,并且在转移性肺癌组织中其mRNA具有较高的阳性检出率,是可能的肺癌转移相关基因。更多还原

【Abstract】 Background and objective Tumor metastasis is a multistep process with many genes involved in.A novel gene MAG-1,identified by suppression subtractive hybridization from lung cancer cells was found to be associated with tumor metastasis.The aims of this work are to investigate the metastasis related effects of MAG-1 on human lung-giant-cell line PLA801,and to compare the expression rate of MAG-1 in cancer tissue from lung cancer patients with different metastatic status.Methods Sense and anti-sense expressing vectors of MAG-1 were constructed and transfected into PLA801C and PLA801D respectively.Colony forming,adherence assay,MTT assay and Transwell experiments were used to evaluate the alterations of clone forming,cell-matrix adherence,proliferating and invasion of the stable transfected cell strains.Western blot was employed to detect the proteins levels of CD44,and MMP-2 in cell strains and mRNA state of MAG-1 in lung cancer tissue from patients with or without pathological metastasis were also analyzed by RT-PCR.Results MAG-1 could increase cell-ECM(Extracellular Matrix) adhesive capacity,promote invasion,enhance cell proliferation and had no effects on clone forming ability of PLA801 cells in soft agar.MAG-1 was also found have positive effects on the protein level of CD44 and MMP-2 in PLA801 cells,and the detection rate of MAG-1 mRNA was much higher in cancer tissue from metastatic patients(7/12) than that in non-metastatic patients(2/12).Conclusion MAG-1 could promote lung cancer metastasis and might be a metastasis associated gene of lung cancer.更多还原