【摘要】 【目的】研究Homer 1a与注意缺陷多动障碍的关系,为临床治疗提供理论依据。【方法】将20只注意缺陷多动障碍动物模型-幼年自发性高血压大鼠(spontaneously hypertensive rat,SHR)随机分为两组,分别给予盐酸哌甲酯(2 mg/kg)或生理盐水,分别利用RT-PCR和Western Blotting技术检测两组大鼠Homer 1a mRNA和蛋白表达差异情况。【结果】盐酸哌甲酯给药组前额叶、海马中Homer 1a mRNA和蛋白的表达较生理盐水对照组显著增加(mRNAt前额叶=2.41,t海马=2.68,蛋白t前额叶=3.21,t海马=4.07,P值均<0.05)。【结论】盐酸哌甲酯单次用药可以引起SHR大鼠前额叶和海马Homer 1a mRNA和蛋白表达的增加,提示Homer 1a可能参与了ADHD的病因及发病机制。更多还原

【Abstract】 【Objective】 To investigate the effect of Homer 1a on attention deficit/hyperactivity disorder(ADHD). 【Methods】 20 animal models of ADHD-spontaneous hypertensive rats(SHR) were divided into two groups(10 rats in each),and given saline(SAL) or methylphenidate(MPH),respectively,to examine the effects of MPH on the expression of Homer 1a using RT-PCR and western blotting,respectively. 【Result】 By RT-PCR and western blotting detection,it was found that the mRNA and protein expressive levels of Homer 1a were increased by MPH compare to SAL(mRNA t=2.41,and 2.68,protein t=3.21,and 4.07,All P<0.05). 【Conclusion】 It is plausible that Homer 1a may be involved in the etiology and pathogenesis of ADHD.更多还原