【摘要】 为研究副鸡嗜血杆菌河北省分离株W菌株致病性及生长特性,以不同剂量对80日龄公鸡进行致病性试验。对W菌株16 h培养物进行100倍稀释,采用微量点板计数法在不同培养时间进行细菌计数以绘制生长曲线。结果表明,试验鸡接菌量在1.11×104cfu/只时发病率可以达到100%,由生长曲线计算W菌株的世代时间为25.33 min。W菌株可能成为研制优良鸡传染性鼻炎疫苗的候选菌株。更多还原

【Abstract】 To study the pathogenicity and the growth property of W isolate,pathogenicity test of W isolate with different dosages was conducted by challenging 80-day-old chichens,16 h culture fluid of W isolate was 100 time diluted,and was taken count of by plat count within different time in order to drow the growth curve.The incidence of test chicken inoculated by 1.11 ×104cfu of W isolate could be 100%.Generation time of W isolate caculated through growth curve was 25.33 min.The W isolate may be used for producing fine vaccine in the future.更多还原