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副鸡嗜血杆菌分离株致病性和生长特性试验

Pathogenicity and Growth Property of Haemophilus paragallinarum Isolate

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【作者】 李杰峰谢春伏刘虹李庆锁梁占军高维芳

【Author】 LI Jie-feng1,XIE Chun-fu1,LIU Hong1,LI Qing-suo1,LIANG Zhan-jun2,GAO Wei-fang3(1.Hebei Provincial Husbandry and Veterinary Research Institute,Baoding,Hebei,071000,China;2.The Bureau of Wuji Raising Livestock,Wuji,Hebei,052460,China;3.The Bureau of Wen’an Raising Livestock,Wen’an,Hebei,065800,China)

【机构】 河北省畜牧兽医研究所河北省无极县畜牧局河北省文安县畜牧局

【摘要】 为研究副鸡嗜血杆菌河北省分离株W菌株致病性及生长特性,以不同剂量对80日龄公鸡进行致病性试验。对W菌株16 h培养物进行100倍稀释,采用微量点板计数法在不同培养时间进行细菌计数以绘制生长曲线。结果表明,试验鸡接菌量在1.11×104cfu/只时发病率可以达到100%,由生长曲线计算W菌株的世代时间为25.33 min。W菌株可能成为研制优良鸡传染性鼻炎疫苗的候选菌株。

【Abstract】 To study the pathogenicity and the growth property of W isolate,pathogenicity test of W isolate with different dosages was conducted by challenging 80-day-old chichens,16 h culture fluid of W isolate was 100 time diluted,and was taken count of by plat count within different time in order to drow the growth curve.The incidence of test chicken inoculated by 1.11 ×104cfu of W isolate could be 100%.Generation time of W isolate caculated through growth curve was 25.33 min.The W isolate may be used for producing fine vaccine in the future.

  • 【文献出处】 动物医学进展 ,Progress in Veterinary Medicine , 编辑部邮箱 ,2009年04期
  • 【分类号】S852.612
  • 【被引频次】6
  • 【下载频次】234
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