【摘要】 目的探讨乌龙丹对慢性脑缺血大鼠学习记忆功能的影响。方法实验分为假手术组、慢性脑缺血模型组、乌龙丹高,中,低剂量组、银杏叶片组。采用双侧颈总动脉永久性结扎建立慢性脑缺血模型;术后第2天开始灌胃给药,连续8周。通过Morris水迷宫和避暗实验检测大鼠的空间学习记忆能力和被动回避学习记忆能力;尼氏染色观察大鼠海马组织结构的变化。结果与模型组比较,乌龙丹给药组和银杏叶组大鼠在Morris水迷宫实验中寻台时间明显缩短(P<0.05,P<0.01);在避暗实验中潜伏期明显延长、错误次数减少(P<0.05,P<0.01)。尼氏染色:模型组海马CA1区神经细胞数量显著减少,排列紊乱,细胞核固缩,与假手术组和乌龙丹高剂量组比较具有明显差异。结论乌龙丹能改善慢性脑缺血大鼠学习记忆能力,其机制可能与保护大鼠海马组织结构有关。更多还原

【Abstract】 Objective To evaluate the effect of Wulongdan, a tradition Chinese medicinal preparation, on the learning and memory abilities of rats with chronic cerebral ischemia and explore the mechanisms. Methods Male rats with chronic cerebral ischemia induced by permanent ligation of the bilateral carotid arteries were randomized into sham-operated group, chronic cerebral ischemia (model) group, and high-, middle-, and low-dose Wulongdan groups and Yinxingye group. The corresponding treatments were administered in the rats 24 h after the operation once daily for 8 consecutive weeks. Morris water maze and step-through tests were performed after 7 weeks of drug administration. The brain tissues were then taken to observe the morphological changes in the hippocampal neurons with Nissl staining by transmission electron microscopy. Results Compared with the model group, the latency of finding the platform in Morris water maze test was significantly shortened (P<0.05 or 0.01), and that in step-through test significantly prolonged (P<0.05 or 0.01) in high-, middle-, and low-dose Wulongdan groups and Yinxingye group. In the model group, Nissl staining of the hippocampal CA1 region visualized obvious pathological changes in the neurons, showing a significant difference from the sham-operated and high-dose Wulongdan groups. Conclusion Wulongdan can enhance the learning and memory abilities of rats with chronic cerebral ischemia possibly through a mechanism in relation to neuronal protection in the hippocampus CA1 region.更多还原