【摘要】 目的探讨中药复方银杏平颤方(GBPR)抗实验性帕金森病(PD)的疗效及作用机制。方法取雄性C57/BL6J小鼠分为正常对照组、模型对照组和中药组。以1-甲基4-苯基-四氢吡啶(MPTP)腹腔注射制作的PD模型小鼠,并给予GBPR灌胃治疗。用地高辛标记的nNOScDNA寡核苷酸探针进行原位杂交,观察GBPR治疗对小鼠脑组织纹状体、黑质中的nNOSmRNA的表达的影响。结果MPTP腹腔注射PD模型小鼠脑纹状体和黑质中可见大量nNOSmRNA的表达;中药GBPR能显著降低脑纹状体、黑质中的nNOSmRNA的表达。结论提示本药有良好的抗NO毒性作用,其作用机制可能与降低脑组织神经型一氧化氮合成酶(nNOS)合成,达到抗氧化损伤效应。更多还原

【Abstract】 Objective To study the effect of the Chinese compound prescription Ginkgo biloba Pingchan Recipe (GBPR) on experimental Parkinson disease (PD) in mice and explore the possible mechanism. Methods Male C57/BL6J mice were divided into normal control, PD model and treatment groups. PD model was established by intraperitoneal injection with 1-methl-4-phenyl-1,2,3,6-tetrahydropyridin (MPTP) in the mice, and in the treatment group, GBPR was administered intragastrically after the injection. The mice were sacrificed 14 and 28 days later, and using in situ hybridization with Digoxin-labeled nNOS cDNA oligonucleotide probe, neuronal nitric oxide synthase (nNOS) mRNA was detected in the striatum and substantia nigra in the brain of mice. Results nNOS mRNA expression was detected in the striatum and substantia nigra of the PD model mice, and GBPR treatment significantly reduced its expressions. Conclusion GBPR has obvious inhibitory effect against the neurotoxicity of NO probably by producing an anti-oxiditive effect through decreasing nNOS synthesis in the brain.更多还原