【摘要】 目的观察多次食管酸灌注对豚鼠气道阻力和气道反应性的影响并探讨其作用机制。方法应用双室体描仪测定对照豚鼠和多次食管酸灌注豚鼠的气道阻力和气道反应性,同时用ELISA法测定豚鼠肺组织、气管、神经节和肺泡灌洗液的P物质(SP)和血管活性肠肽(VIP)含量。结果多次食管灌酸组豚鼠第15天灌酸后的气道阻力增长率与正常对照组比较差异有统计学意义(P<0.05),4个时间点气道反应性的差异无统计学意义。多次食管酸灌注可增加豚鼠肺组织、气管、结状神经节、支气管肺泡灌洗液(BALF)中SP含量。结状神经节SP含量与气管中SP含量呈正相关。正常对照组、多次灌生理盐水组、多次灌酸组豚鼠肺组织、气管、神经节、BALF的VIP含量两两比较差异无统计学意义。结论多次食管灌注盐酸可以增加豚鼠气道阻力但不能改变其气道反应性,其原因可能与迷走神经介导的食管-支气管反射引起气道神经末梢释放SP有关。更多还原

【Abstract】 Objective To observe the effect of repeated esophageal acid infusion on specific airway resistance(sRaw) and airway reactivity in the guinea pigs and explore the mechanism.Methods sRaw and airway reactivity were measured by double-chamber plethysmography in normal control group(group N),saline control group(group NS),and repeated acid irrigation group(group H).The initial measurement was used as the baseline sRaw and airway reactivity(1d1),and 2 h after the initial measurement,sRaw and airway reactivity were measured again(1d2).Similarly,such measurements were repeated on the 15th day for all the guinea pigs(15d1,15d2) with a 2-h interval.The content of Substance P(SP) and vasoactive intestinal peptide(VIP) in lung tissue,trachea,BALF and ganglion were detected by ELISA.Results The percent change of sRaw,(15d2-1d1)/1d1 in group H was significantly higher than that in group N.The differences in the airway reactivity of the group N,group NS,and group H were not statistically significant.The SP content in the lung,trachea,ganglion and bronchoalveolar lavage fluid(BALF) in group H was significantly higher than those in group N.The SP content in ganglion showed a significant positive correlation to that in the trachea.No significant differences were found in the VIP content in the lung,trachea,ganglion or BALF between the groups.Conclusion Repeated esophageal acid infusion increases the airway resistance,but not the airway reactivity in normal guinea pigs.SP may be involved in development of high sRaw through the esophageal-tracheobronchial reflex.更多还原