【摘要】 目的建立妇安口服液的质量标准。方法高效毛细管电泳法对处方中主要药材蒲公英中的阿魏酸进行含量测定,并采用薄层色谱鉴别法鉴别蒲公英和柴胡。高效毛细管电泳条件:石英毛细管柱(70μm×60cm),运行缓冲液为20mmol/L硼砂溶液(pH=9.18),分离电压12kV,真空进样时间5s,柱温25℃,紫外检测波长313nm。结果薄层色谱法结果可见清晰的斑点,重复性好,阴性样品无干扰。高效毛细管电泳结果显示:阿魏酸在8～40μg/ml范围内呈良好线性关系,Y=360.5Х-207.4(r=0.9997,n=5)。平均回收率均大于98%;RSD均小于3%(n=3)。结论本方法简便可靠,专属性、重现性好,为妇安口服液质量控制提供了可靠的指标。更多还原

【Abstract】 Objective To establish a quality control method of Fuan oral liquid.Methods High-performance capillary electrophoresis(HPCE) was used to determine the content of feruild acid in Herba taraxaci,and thin-layer chromatography(TLC) was performed to identify Herba taraxaci and Bupleurum chinense.The condition of HPCE was optimized with a fuse silica capillary tube(70 μm×60 cm) and 20 mmol/L sodium tetraborate buffer(pH9.18) at a constant voltage of 12 kV and temperature at 25 ℃,with the detection wavelength at 313 nm.Results Clear spots were displayed on TLC.The calibration curve was linear in the range of 8-40 μg/ml for ferulic acid(Y=360.5Х-207.4,r=0.9997,n=5).The average recovery rate exceeded 95% with a RSD<3%(n=3).Conclusion This method is simple and specific with a good reproducibility for quality control of Fuan oral liquid.更多还原