【摘要】 目的研究刺五加体外诱导骨髓基质细胞(MSCs)分化为神经元样细胞。方法采用全骨髓贴壁筛选法分离培养MSCs。含刺五加的无血清DMEM/F12诱导MSCs的分化。间接免疫荧光法、Western blot及RT-PCR检测巢蛋白(nestin)、β-Tubulin III及GFAP蛋白和mRNA的表达。结果MSCs在体外传代扩增至第3代,CD44、CD54表达阳性已达90%以上,而CD14表达阳性下降至2.37%。刺五加诱导后,MSCs胞体收缩,突起伸出,类似神经元;免疫荧光、Western blot及RT-PCR均显示刺五加诱导后的细胞nestin、β-Tubulin III蛋白和mRNA表达阳性,而GFAP表达阴性。结论刺五加可以在体外诱导MSCs分化为神经元样细胞。更多还原

【Abstract】 Objective To study the effect of the traditional Chinese herbal drug Ciwujia in inducing the differentiation of marrow stromal cells(MSCs) into neuron-like cells.Methods Rat MSCs isolated from the whole bone marrow were amplified by adherent culture in vitro and induced to differentiate into neuron-like cells using serum-free DMEM/F12 containing Ciwujia.The protein and mRNA expressions of nestin,β-Tubulin III and glial fibrillary acidicprotein(GFAP) in the differentiated cells were detected by indirect immunofluorescence method,Western blotting and RT-PCR.Results The third-passage MSCs showed positive expression rates for CD44 and CD54 beyond 90% with decreased CD14 expression rate to 2.37%.Induction by Ciwujia of the MSCs resulted in cell body shrinkage and protrusion of the cell processes resembling those of neurons.The differentiated cells were positive for nestin and β-Tubulin III expression and negative for GFAP as shown by immunofluorescence assay,Western blotting and RT-PCR.Conclusion Ciwujia can induce the differentiation of rat MSCs into neuron-like cells in vitro.更多还原