【摘要】 目的以大鼠为对象,初步探讨华支睾吸虫成虫分泌/排泄抗原(CsESAs)在华支睾吸虫致肝纤维化过程中的作用和可能机制。方法无菌培养华支睾吸虫成虫,收集CsESAs;腹腔注射CsESAs,Masson染色观察CsESAs对大鼠肝脏胶原纤维增生的影响,HE染色和组织免疫荧光法检测α-SMA(α-smooth muscle actin)的表达以观察CsESAs对大鼠肝星状细胞增生和活化的影响;ELISA法检测实验动物血清中抗CsESAs抗体滴度以探讨抗原-抗体复合物的致病作用。结果腹腔注射CsESAs,大鼠肝脏表现出明显的纤维化、肝星状细胞增生和活化,但大鼠肝纤维化程度与注射的CsESAs量有关而与大鼠血清中抗CsESAs抗体存在量无关。结论CsESAs可以导致肝星状细胞活化和肝纤维化的发生,但抗原-抗体复合物不是CsESAs引起肝星状细胞活化的主要途径。更多还原

【Abstract】 Objective To investigate the role of excretory/secretory antigens from Clonorchis sinensis(CsESAs) in hepatic fibrosis induced by C.sinensis infection in rats and explore the possible mechanism.Methods CsESAs was collected from adult C.sinensis cultured in sterile condition for 12 h and injected intraperitoneally in Wistar rats.Masson staining was used to observe the changes in the hepatic collagen fiber after the injection.HE staining and immunofluorescence staining were performed to detect the expression of α-smooth muscle actin(α-SMA) to examin the proliferation and the activity of hepatic stellate cells.The specific antibody titer of CsESAs was determined using enzyme-linked immunosorbent assay to investigate the role of the antigen-antibody complex in the development of hepatic fibrosis.Results After intraperitoneal injection of CsESAs,obvious hepatic fibrosis and hepatic stellate cell proliferation and activation were observed in the rat livers.The severity of the hepatic fibrosis was associated with the dose of CsESAs injected,whereas the titer of the specific antibody against CsESAs showed no direct relation to the hepatic fibrosis.Conclusion Intraperitoneal injection of CsESAs can cause hepatic stellate cell activation and hepatic fibrosis in rats,but the antigen-antibody complex does not seem to play the key role in the activation of the hepatic stellate cells.更多还原