【摘要】 目的探讨Caspase-3基因表达对人鼻咽癌细胞CNE2的凋亡诱导作用。方法应用重组技术将重构型Caspase-3基因亚克隆至带有报告基因的真核表达载体pEGFP中,脂质体介导转染人鼻咽癌细胞CNE2通过RT-PCR方法检测转染后Caspase-3基因的表达;荧光显微镜、电镜观察细胞形态学变化;MTT法检测转染Caspase-3基因对CNE2细胞生长的影响。结果RT-PCR方法证实重构型Caspase-3基因可在CNE2细胞内表达,形态学观察显示转染组细胞较对照组出现明显细胞凋亡特征,结果表明Caspase-3对CNE2的细胞生长有抑制作用。结论重构型Caspase-3对CNE2细胞的生长有抑制作用。更多还原

【Abstract】 Objective To investigate the apoptosis-inducing effect of caspase-3 on human nasopharyngeal carcinoma cells(CNE2).Methods Recombinant caspase-3 was subcloned into the eukaryotic expression vector PEGFP-C1 containing the reporter gene using DNA recombinant technique.CNE2 cells were transfected with the recombinant caspase-3 gene via lipofectamine 2000 and the expression of caspase-3 mRNA was detected by reverse transcription-polymerase chain reaction.The cell morphological changes were observed under fluorescence microscope and electron microscope and the cell survival rate after the transfection was assessed by MTT assay.Results Transfection with the recombinant caspase-3 gene induced significant apoptosis in CNE2 cells, which exhibited obvious morphological changes typical of apoptotic cells.Conclusion The recombinant caspase-3 gene can inhibit the growth and effectively induce apoptosis of CNE2 cells.更多还原