【摘要】 目的探讨Tiam-1蛋白与鼻咽癌生物学行为的关系。方法用免疫组化方法检测60例不同临床分期人鼻咽癌组织、20例鼻咽粘膜慢性炎症组织以及6例裸鼠人鼻咽癌转移细胞株模型标本中Tiam-1蛋白的表达。结果Tiam-1蛋白在鼻咽癌组织中的阳性表达率63.33%高于慢性炎症组36.67%(χ2=20.429,P=0.001);Tiam-1蛋白在鼻咽癌组的平均表达得分(2.9167±1.3057)高于鼻咽粘膜慢性炎组(0.7000±0.9234)(t=7.0162,P=0.0000)。Tiam-1蛋白表达在临床T分期之间没有统计学差异(F=2.36,P=0.0811),在有无淋巴结和器官转移的肿瘤组织中均有统计学意义(P=0.0001)。在具有转移特性的人源性鼻咽癌细胞株裸鼠模型肿瘤组织中Tiam-1蛋白均呈高表达。结论Tiam-1与鼻咽癌浸润转移密切相关,提示Tiam-1蛋白可能是鼻咽癌的重要促侵袭转移因子。更多还原

【Abstract】 Objective To investigate the relationship between the protein expression of T-lymphoma invasion and metastasis gene 1(Tiam-1) and the biological behaviors of nasopharyngeal carcinoma(NPC).Methods Immunohistochemistry was performed to detect the expressions of Tiam-1 protein in 60 specimens of NPC tissue, 20 specimens of chronic nasopharyngitis(CN) tissue, and 6 tumor tissues from nude mice inoculated with metastatic human NPC cells.Results The positivity rate and average score for Tiam-1 expression were significantly higher in NPC tissues than in CN tissue(63.33% vs 36.67%, 2.9167± 1.3057 vs 0.7000±0.9234;χ2=20.429, P=0.001;t=7.0162, P=0.0000, respectively).No difference was found in Tiam-1 expression among NPC patients in different T stages(F=2.36, P=0.0811), while the expression differed significantly between the patients with lymph node metastasis and those without metastasis, and also between patients with organ metastasis and those without(P=0.0001).High Tiam-1 expressions were found in the tumor tissues in nude mice inoculated with metastatic NPC cells.Conclusion Tiam-1expression is closely associated with the invasiveness and metastasis of NPC, indicating that Tiam-1 is an important factor that promotes the invasion and metastasis of NPC.更多还原