【摘要】 本文旨在研究谷胱甘肽(glutathione,GSH)对育肥羊生长性能及生长激素/胰岛素样生长因子-Ⅰ(GH/IGF-Ⅰ)轴的影响。试验采用单因素随机区组设计,将12只3月龄东北细毛羊与德国肉用美利奴羊杂交一代育肥羊分为3组,每组4个重复,每组羊公母各占1/2。对照组饲喂基础日粮,试验组1和试验组2分别在基础日粮的基础上添加500和800 mg/kg的GSH,试验期为60 d。测定谷胱甘肽对育肥羊生长性能和血清中GH和IGF-Ⅰ浓度及组织中IGF-Ⅰ mRNA表达量的影响。结果表明:日粮中添加GSH显著提高了试验组育肥羊的日增重(P<0.05),显著降低了试验组育肥羊的料重比(P<0.05),但对育肥羊的日采食量无显著影响(P>0.05);试验20、40和60 d时,试验组育肥羊血清中的生长激素(GH)和胰岛素样生长因子-Ⅰ(IGF-Ⅰ)水平显著高于对照组(P<0.05);与对照组相比,试验组育肥羊肝脏和背最长肌中IGF-Ⅰ mRNA的表达量有所提高,当GSH添加量达到800 mg/kg时,育肥羊背最长肌中IGF-Ⅰ mRNA的表达量显著高于对照组(P<0.05)。因此,本试验初步得出,日粮中添加GSH促进了肝脏IGF-Ⅰ的分泌,提高了肌肉中IGF-Ⅰ mRNA的表达量,提高血清中GH与IGF-Ⅰ浓度,从而促进了育肥羊的生长。更多还原

【Abstract】 This experiment was conducted to study the effects of glutathione (GSH) on growth performance and growth hormone /insulin-like growth factor- I axis in fattening sheep. Twelve sheep aged at three months were randomly allocated into three groups: control (basal diets), treatments 1 (basal diets supplemented with 500 mg/kg GSH) and 2 (basal diets supplemented with 800 mg/kg GSH), with 4 replicates in each treatment. The trial lasted for 60 days. Effects of dietary supplementation of GSH on growth, GH and IGF-Ⅰ levels in serum and IGF-Ⅰ mRNA expression in tissues of fattening sheep were studied. The results showed that the average daily gain (ADG) and the ratio of feed to gain (F/G) was decreased significantly (P<0. 05) for sheep supplemented with GSH, and there were no significant differences about average daily feed intake (ADFI) (F>0. 05) in all groups. The serum GH and IGF-Ⅰ levels of sheep in all GSH groups were significantly higher than those in control group (P<0. 05) at trial period 20, 40 and 60 d. Compared with control group, IGF- Ⅰ mRNA expression in liver and muscle of sheep in all GSH groups were increased, and IGF- Ⅰ mRNA expression in muscle of treatment 2 was increased significantly (P<0. 05). These results indicated that dietary GSH supplementation in diets could stimulate the secretion of IGF- Ⅰ in liver and the expression of IGF- Ⅰ mRNA in muscles, increase the serum GH and IGF-Ⅰ levels, and thereby promote the growth of fattening sheep. [Chinese Journal of Animal Nutrition, 2009,21(3) :312-318 ]更多还原