【摘要】 目的:研究纤维蛋白原(Fg)Bβ-249C/T,Bcl-1G/A的基因多态性、血浆Fg浓度、分子聚合活性等指标与脑梗死(CI)类型的关系.方法:采用病例对照研究方法,选取主干支脑梗死(MCI)患者54例,穿通支脑梗死(PCI)患者106例,正常对照组160例,应用聚合酶链反应-限制性酶切法进行Bβ-249C/T,Bcl-1G/A位点的基因多态性分析.抽取患者清晨空腹静脉血测定血糖等4项生化指标和血浆Fg浓度,Fg单体聚合反应速率(FMPV),最大光密度(Amax),FMPV/Amax等反映Fg分子聚合功能的参数,并进行体检.结果:BβBcl-1G/A的A等位基因和GA,AA基因型在MCI组均高于对照组(P<0.05),FgBβ-249C/T等位基因和各基因型在MCI组,PCI组和对照组组间的分布频率差异无显著性.MCI组Bβ-249C/T的CT+TT型Fg浓度(4.04±0.88)g/L,FMPV(0.72±0.12)均低于CC型Fg浓度(5.01±0.43)g/L,FMPV(0.83±0.06)(P<0.05).对照组BβBcl-1G/A的GA+AA型Fg浓度(4.03±0.90)g/L高于GG型(3.69±0.95)g/L(P<0.05);PCI组BβBcl-1G/A的GA+AA型FMPV(0.74±0.11)高于GG型(0.69±0.11)(P<0.05).结论:Bβ-249C/T基因多态性可能是影响血浆FMPV功能表达的重要位点,BβBcl-1GA,AA基因型可能仅使血浆纤维蛋白单体聚合功能表达增强而诱发PCI.更多还原

【Abstract】 AIM:To explore the relation of Beta-fibrinogen(Fg)gene-249C/T and Bcl-1G/A polymorphisms,the Fg indexes of Fg concentration,molecular polymerized function and the type of cerebral infarction.METHODS:A case-control study was used to analyze 54 patients with main-trunk cerebral infarction(MCI),106 patients with penetrating cerebral infarction(PCI)and 160 normal control;Gene polymorphisms were analyzed by polymerase chain reaction-restriction fragment length polymorphism;All sample with empty stomach should be drawn venous blood on early morning to determine 4 biochemistry indexes and the parameters illustrated Fg molecular reactivity,such as:Fg concentration,fibrin monomer polymerize velocity(FMPV),absorbance maximum(Amax),FMPV/Amax;All sample were examined by trained interviewers.RESULTS:The frequency of A Bcl-1 allele,GA+AA genotype in the group of MCI were higher than that of normal control(P<0.05),No distinction of the frequency of Bβ-249C/T’s genotype and allele between MCI,PCI and normal control were found.In the group of MCI,Fg concentration and FMPV of Bβ-249C/T CT+TT genotype were(4.04±0.88)g/L and(0.72±0.12)by turns,while these of CC genotype were(5.01±0.43)g/L and(0.83±0.06).Fg concentration and FMPV of Bβ-249C/T CT+TT genotype was lower than that of CC genotype(P<0.05).In the group of normal control,Fg concentration of BβBcl-1G/A GA+AA genotype was(4.03±0.90)g/L,which was higher than GG genotype’s Fg concentration that was(3.69±0.95)g/L(P<0.05).In the group of PCI,FMPV of BβBcl-1G/A GA+AA genotype was(0.74±0.11),which was higher than GG genotype’s FMPV that was(0.69±0.11)(P<0.05).CONCLUSION:Bβ-249C/T was probably the important sites of the function express of FMPV,BβBcl-1GA,AA genotype could induce PCI by increasing the function expression of fibrin monomer polymerize function.更多还原