【摘要】 目的:比较小鼠真皮间充质干细胞(mdMSCs)和人胚胎真皮间充质干细胞(hdMSCs)对人皮肤成纤维细胞(hsFb)组织修复作用的影响,探讨异种属真皮间充质干细胞(MSCs)促进hsFb组织修复的可靠性.方法:采用低血清培养基,以消化—贴壁—传代法体外培养、鉴定mdMSCs和hdM-SCs,并与体外分离培养的hsFb于transwell培养体系中共培养,采用样本碱水解法和ELISA法分别检测第1,4日培养上清液中羟脯氨酸(Hyp)和转化生长因子-β1(TGF-β1)的变化.结果:不同种属MSCs(mdMSCs/hdMSCs)对共培养上清液Hyp含量的影响差异(F=0.174)以及不同处理组(即不同细胞密度MSCs)培养上清液Hyp含量差异(F=1.304)均无显著性.不同时间(即第1和第4日)共培养上清液Hyp含量差异具有统计学意义(F=8.712,P<0.01).不同种属MSCs(mdMSCs/hdMSCs)对上清液TGF-β1的含量影响差异(F=0.062)以及不同处理组(即不同细胞密度MSCs)培养上清液TGF-β1含量差异(F=1.991)均无显著性.不同时间(即第1和第4日)培养上清液TGF-β1含量差异具有统计学意义(F=0.699,P<0.05).结论:不同来源真皮MSCs与hsFb共培养对胶原分泌和TGF-β1表达无影响,组织修复过程中,异种属真皮MSCs的诱导是可靠的.更多还原

【Abstract】 AIM:To compare the tissue repair effect of mouse dermis-derived mesenchymal stem cells(mdMSCs)and human dermis-derived MSCs(hdMSCs)on normal human skin fibroblast(hsFb) and to explore the reliability of heterogeneously dermis-derived MSCs in tissue repair.METHODS:mdMSCs and hdMSCs were isolated and cultivated by tissue digested-adherent-passage in low-serum medium and the changes of hydroxyproline(Hyp) and TGF-β1 were determined in co-culture system of mdMSCs or hdMSCs and hsFb on day 1 and 4 by samples basic hydrolysis and ELISA respectively.RESULTS:No significant difference was found in the contents of Hyp between groups of different MSCs(F=0.174) or between groups of different cell density(F=1.304).The contents of Hyp were significantly different between day 1 and day 4(F=8.712,P<0.01).The level of TGF-β1 was not significantly different between groups of different MSCs(F=0.062) or between groups of different cell density(F=1.991).The level of TGF-β1 was significantly different between day 1 and day 4(F=0.699,P<0.05).CONCLUSION:Dermal MSCs of different origin(mouse or human) would not affect the levels of collagen and TGF-β1 when cultivated with hsFb.It is reliable for heterogeneously dermis-derived MSCs to promote tissue repair on hsFb.更多还原