【摘要】 目的:探讨ABCA1基因13148C/T单核苷酸多态性与中国甘肃地区汉族人群2型糖尿病及脂质代谢的关系.方法:采用巢式多聚酶链反应-限制性片段长度多态性(nested-PCR-RFLP)技术检测148例2型糖尿病患者和114例健康对照组ABCA1基因13148C/T单核苷酸多态性.结果:①2型糖尿病组CC型频率低于对照组(P<0.05),而TT型频率高于对照组(P<0.05),等位基因频率在两组间的差异有统计学意义(P<0.05).②2型糖尿病组的体质量指数、空腹血糖、收缩压、总胆固醇、三酰甘油、低密度脂蛋白胆固醇、空腹胰岛素、HOMA胰岛素抵抗指数、糖化血红蛋白高于对照组,而高密度脂蛋白胆固醇低于对照组,差异有统计学意义(P<0.05).③2型糖尿病组三酰甘油、HOMA胰岛素抵抗指数在CC型、CT型、TT型之间依次递增,差异无统计学意义,高密度脂蛋白胆固醇(HDL-C)水平在CC型、CT型、TT型之间依次递减,TT型的HDL-C水平明显低于CC型,差异有统计学意义(P<0.05).结论:2型糖尿病患者脂质代谢发生紊乱且与胰岛素抵抗、肥胖、高血压密切相关;ABCA1基因13148C/T多态性与2型糖尿病相关,并与其血脂代谢相关;T等位基因可能是2型糖尿病的易感等位基因,TT基因型可能是2型糖尿病的易感基因型.更多还原

【Abstract】 AIM:To observe whether the ABCA1 gene 13148C/T single-nucleotide polymorphism is associated with type 2 diabetes mellitus and lipid metabolism. METHODS: The genotype and allele frequencies of ABCA1 13148C/T genetic variation were assayed by nest-polymerase chain reaction-restriction fragment length polymorphism (nest-PCR-RFLP). A case-control study was conducted in 148 patients with type 2 diabetes mellitus and 114 healthy controls. RESULTS: The CC genotype frequency in type 2 diabetes mellitus group was lower than that in healthy controls (P<0.05) while the TT genotype frequency in type 2 diabetes mellitus group was higher than that in healthy controls (P<0.05), with significant difference in the frequency of the T allele between DM and CON group (P<0.05). The BMI, FPG, SBP, TC, TG, LDL-C, Fins, HOMA-IR and HbA1c levels in type 2 diabetes mellitus group were higher than those in healthy controls (P<0.05), while HDL-C in type 2 diabetes mellitus group was lower than that in healthy controls(P<0.05). The level of TG and HOMA-IR gradually increased while that of HDL-C gradually decreased in genotype of CC, CT and TT. HDL-C level of TT genotype was significantly lower than that of CC genotype (P<0.05). CONCLUSION: There exists an association between ABCA1 13148C/T polymorphism and type 2 diabetes mellitus, which is associated, to some extent, with lipid metabolism. T allele and TT genotype appear to be risk factors for type 2 diabetes mellitus in Han nationality in Gansu Province of China.更多还原