【摘要】 目的:采用高效液相色谱(HPLC)建立同时测定健康成人血清中叶黄素和β-胡萝卜素的方法.方法:血清经蛋白沉淀,正己烷萃取,氮气吹干及流动相溶解等预处理过程后,进行高效液相色谱分析.采用Discovery C18色谱柱(150 mm×4.6 mm.id.,5μm),以乙腈-甲醇为流动相进行梯度洗脱,检测波长为450 nm.结果:叶黄素和β-胡萝卜素在0.1～10.0 mg/L范围内与峰面积呈良好线性关系,相关系数r分别为0.9996,0.9985(P<0.05).叶黄素和β-胡萝卜素的平均加样回收率在93.0%～103.9%之间,相对标准偏差(RSD)分别为1.19%和2.70%,分析周期在25 min以内.结论:该方法灵敏度高,重现性好,是定量测定血清中叶黄素和β-胡萝卜素定量测定的可靠方法之一.更多还原

【Abstract】 AIM: To develop a method for the simultaneous determination of serum lutein and β-carotene levels in healthy Chinese subjects by high performance liquid chromatography(HPLC).METHODS: Lutein and β-carotene were extracted by hexane after protein precipitation.The organic layer was evaporated under N2 and the residue was dissolved in mobile phase.Serum lutein and β-carotene levels were determined by HPLC.Discovery C18 column(150 mm×4.6 mm i.d.,5 μm) was employed.The mobile phase consisted of acetonitrile and methanol with the gradient elution.The detection wavelength was set at 450 nm.RESULTS: Lutein and β-carotene showed good linear relationships in the range of 0.1-10.0 mg/L,r=0.9996 and r=0.9985(P<0.05).The recoveries of lutein and β-carotene were 93.0%-103.9%,and RSDs were 1.19%-2.70%.The separation was completed within 25 min.CONCLUSION: This established method is accurate and reproducible.It can be used for the analysis of lutein and β-carotene in serum.更多还原