【摘要】 目的:探讨以c-erbB2 mRNA为靶点的反义寡脱氧核苷酸(ASODN)联合紫杉醇对乳腺癌SK-Br-3细胞的抑制作用.方法:实验分为7组:ASODN组,紫杉醇组,赫赛汀组,表阿霉素组,ASODN+紫杉醇组,赫赛汀+紫杉醇组,表阿霉素+紫杉醇组.分别处理乳腺癌SK-Br-3细胞72 h,MTT法测定细胞抑制率,中效原理判定药物联合应用的效果,WesternBlot检测细胞c-erbB2,Bcl-2蛋白表达水平.结果:各组中SK-Br-3细胞的抑制率均随药物浓度的增加而增大;紫杉醇的中效浓度紫杉醇组为0.14μmol/L;ASODN+紫杉醇组为0.07μmol/L,其合用指数(CI)<1,为协同作用.ASODN+紫杉醇组c-erbB2,Bcl-2蛋白表达明显低于紫杉醇组,两组相比较,差异具有统计学意义(P<0.05).结论:ASODN可明显增强紫杉醇对乳腺癌SK-Br-3细胞的抑制效应,可明显降低紫杉醇的用药量.更多还原

【Abstract】 AIM: To explore the inhibitory effect on human breast cancer SK-Br-3 cells treated with c-erbB2 antisense oligodeoxynucleotide(ASODN) combined with taxol.METHODS: SK-Br-3 cells were treated with ASODN,taxol,herceptin,Epirubicin,ASODN combined with taxol,herceptin combined with taxol,and Epirubicin combined with taxol for 72 h respectively.The inhibitory effect was detected by MTT assay.The drug’s interactional effect was determined by median-effect principle.The expression of c-erbB2 and Bcl-2 was detected by western blot in SK-Br-3 cells.RESULTS: The inhibition ratio of each group increased while the increased concentration of drugs used.The median concentration of taxol was 0.14 μmol/L in taxol group and 0.07 μmol/L in ASODN combined taxol group respectively.The combination index(CI) of ASODN combined with taxol group was always less than 1,indicating that there was some synergistic effects between ASODN and taxol.The expression of c-erbB2 and Bcl-2 proteins was significantly lower in ASODN+taxol group than that in other groups and the difference was statistical significance(P<0.05).CONCLUSION: ASODN significantly enhances the inhibitory effect of taxol on human breast carcinoma SK-Br-3 cells and markedly lowers the taxol’s dosage of administration.更多还原