【摘要】 目的观察不同浓度白介素-8(interleukin-8,IL-8)对体外培养兔外周血内皮祖细胞(endothelial progenitor cells,EPCs)生物学功能的影响,探讨趋化因子IL-8/CXCR2轴对EPCs血管生成功能的作用。方法密度梯度离心法获取兔外周血单个核细胞(mononuclear cells,MNCs),接种至纤维连接蛋白(fibronectin,FN)包被的培养板上,培养7～10d后收集贴壁细胞鉴定EPCs,并用不同浓度IL-8的培养基继续培养24h后采用MTT比色法、改良的Boyden小室实验,观察EPCs的迁移和增殖能力;采用RT-PCR、Western blot方法检测CXCR2、VEGF mRNA及蛋白表达水平。结果IL-8显著增加兔外周血EPCs的增殖和迁移能力(P<0.05),且随着IL-8浓度升高而增强。IL-8促进CXCR2膜受体和VEGF的表达,且随着IL-8浓度增加而增加。结论IL-8/CXCR2轴能显著促进EPCs的迁移、增殖及VEGF的表达。更多还原

【Abstract】 Objective To investigate the proliferation,migration and VEGF expression of rabbit peripheral blood endothelial progenitor cells (EPCs) after the treatment of interleukin-8 (IL-8) at different concentrations.Methods Total mononuclear cells (MNCs)were isolated from rabbit peripheral blood by density gradient centrifugation,and then the cells were plated on fibronectin-coated culture dishes.After cultivation for 7 d,the attached cells were incubated with IL-8 in a series of concentrations for 24 h.The proliferation and migration activities of EPCs were measured with MTT assay and modified Boyden chamber assay,and reverse transcription (RT)-polymerase chain reaction (PCR) and Western blot analysis were used to detect the mRNA and protein expressions of CXCR2 and VEGF of EPCs.Results The proliferation,migration and CXCR2 and VEGF expressions of EPCs were regulated by IL-8,and enhanced with the increasing concentration of IL-8.Conclusion The IL-8/CXCR2 axis can significantly promote the EPCs proliferation,migration,and VGEF expression.更多还原