【摘要】 目的观察海藻酸钠-壳聚糖-海藻酸钠(alginate-chitosan-alginate,ACA)微囊、海藻酸钠-多聚赖氨酸-海藻酸钠(alginate-polylysine-alginate,APA)微囊的强度、通透性及生物相容性,评价ACA、APA 2种微囊在细胞移植中作为运载工具的可行性。方法分别制备ACA微囊和APA微囊,采用37℃摇床振荡对这2种微囊机械强度进行比较研究。以胰蛋白酶、异硫氰酸荧光素标记的IgG、牛血清白蛋白BSA来观察囊膜的通透性。用ACA、APA微囊包封293细胞,植入新西兰大白兔眶周、大鼠腹腔,回收微囊,观察囊内细胞存活情况及微囊的生物相容性。结果ACA和APA微囊囊膜的机械强度良好,所制备的APA微囊允许相对分子质量为22×103的胰蛋白酶、相对分子质量为66×103的牛血清白蛋白通过,2种微囊均限制相对分子质量为150×103的大分子免疫球蛋白透过。回收移植10周的ACA、APA细胞微囊,其形态保持不变,回收微囊化的293细胞在体外进行再次培养,细胞形态正常,移植后大白兔眼组织未见明显炎症改变,大鼠的重要器官(心、肝、脾、肺、肾)无明显的病理改变。结论ACA、APA微囊是细胞移植过程中包裹、保护细胞的合适载体。更多还原

【Abstract】 Objective To investigate the mechanical strength,permeability and biocompatibility of the microcapsules made of alginate-chitosan-alginate(ACA) or alginate-polylysine-alginate(APA),and evaluate the feasibility of ACA and APA microcapsules as carriers of cell transplantation.Methods The ACA microcapsules and the APA microcapsules were prepared separately based on the microencapsulated 293 cells and alginate solution.The mechanical strength of the microcapsules was measured in the shaking apparatus at 37 ℃.The permeability of both microcapsules was observed in present of some proteins,such as trypsin,immunoglobulin IgG and bovine serum albumin by fluorescent microscopy.The ACA and APA microcapsules entrapping 293 cells were transplanted to the periorbit of New Zealand White rabbit and the abdominal cavity of SD rats.After microcapsules were retrieved termly,the viability of encapsulated cells and the biocompatibility of microcapsules were also examined.Results Both the membranes of ACA and APA microcapsules were electrolyte complexes,with enough mechanic strength.APA membranes were permeable to small molecule proteins such as trypsin,bovine serum albumin and impermeable to large molecule proteins such as immunoglobulin.The ACA and APA microcapsules remained intact after transplantation for 10 weeks.The retrieved 293 cells grew well after reculturing.After microcapsules entrapping 293 cells were retrieved.No sign for inflammation and pathological change was found in the anterior or posterior segment of New Zealand White rabbit and the important organs of SD mice.Conclusion The obtained results indicate that both the ACA microcapsules and APA microcapsules are proper cell carriers in transplantation.更多还原