【摘要】 对从北美海蓬子中分离的Na+/H+逆向转运蛋白基因SbNHX1进行了耐盐性及功能结构域分析.利用套叠PCR技术去除SbNHX1基因C末端162个核苷酸,得到SbNHX1-C基因,然后将SbNHX1、SbNHX1-C和拟南芥Na+/H+逆向转运蛋白基因AtNHX1分别插入pET22b(+)表达载体,转化大肠杆菌B菌株,进行各种金属盐离子胁迫分析.结果表明,北美海蓬子Na+/H+逆向转运蛋白基因SbNHX1只对Na+、K+离子有抗性,且耐盐性强于拟南芥Na+/H+逆向转运蛋白基因AtNHX1.缺失C末端的SbNHX1-C基因对Na+、K+离子胁迫无抗性,说明北美海蓬子Na+/H+逆向转运蛋白基因SbNHX1的耐盐作用与该基因C末端1353bp至1514bp的序列密切相关.更多还原

【Abstract】 In this report,we analyzed salt-tolerance and functional domain of Na+/H+ antiporter gene SbNHX1 separated from Salicornia bigelovii Torr..SbNHX1 gene,the mutated SbNHX1-C gene,which cut off 162 bp at C-terminal of SbNHX1 by overlap extensing PCR,and Arabidopsis Na+/H+ antiporter gene AtNHX1 were inserted into the pET22b(+) and transformed to E.coli B separately.The salt-toleranc of some alkali cation has been analyzed.The result indicated that SbNHX1 could only resist to Na+ and K+,which the tolerance was higher than that of AtNHX1.SbNHX1-C gene,which deleted C-terminal,could not resist to Na+ and K+.It is deduced that the sequence from 1 353～1 514 bp in SbNHX1 gene plays an important role for SbNHX1 to Na+ and K+ tolerances.更多还原