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不同还原性巯基氨基酸对人血管平滑肌细胞增殖的影响

A Comparative Study of the Proliferation of Smooth Muscle Cells by Reduced Thiol Amino

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【作者】 苏娟秦燕田昆仑王树人

【Author】 SU Juan1,QIN Yan1,TIAN Kunlun1,WANG Shuren2(1.Collgeg of Basic Medicine,Dali University,Dali,Yunnan 671000,China;2.West China School of Preclinical and Forensic Medicine,Sichuan University,Chengdu,Sichuan 610041,China)

【机构】 大理学院基础医学院四川大学华西基础医学与法医学院

【摘要】 目的:观察三种还原性巯基氨基酸对人血管平滑肌细胞增殖的影响。方法:采用组织贴块法体外培养人脐静脉血管平滑肌细胞(VSMCs),在培养液中分别加入三种不同浓度的还原性巯基氨基酸(Hcy,Cys,GSH)对VSMCs进行刺激,24h后同MTT比色法测定细胞的活力,3H-TdR测定细胞的增殖率。结果:随浓度增加,Hcy和Cys明显促进VSMCs增殖,Hcy的作用略强于Cys;GSH只有在极高浓度时(1 000μmol/L)才显著促进VSMCs增殖。结论:极高浓度的Hcy、Cys、GSH均可促进VSMCs增殖。

【Abstract】 Objective:This study was intended to observe the effects of three redox thiol amino acids(Hcy,Cys and GSH)on the proliferation of human vascular smooth muscle cells(HVSMCs) cultured in vitro.Methods: HVSMCs were cultured in vitro and promoted to Hcy,Cys and GSH,to observe the degree of HVSMCs proliferation by MTT assay and 3H-thymidine(3H-TdR) incorporation test.Results:Hcy and Cys both significantly increased the proliferation of HVSMCs,but the Hcy had much stronger potency.High concentration of GSH(1000μmol/L) significantly increased the proliferation of HVSMCs.Conclusion:High concentration of Hcy,Cys and GSH could induce the proliferation of HVSMCs.

【基金】 高等学校博士学科点专项科研基金资助项目(200506100SO)
  • 【文献出处】 大理学院学报 ,Journal of Dali University , 编辑部邮箱 ,2009年02期
  • 【分类号】R543
  • 【被引频次】1
  • 【下载频次】121
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