【摘要】 将筛选自仿刺参Apostichopus japonicus肠道的乳酸菌L-2、芽孢杆菌K-3和芽孢杆菌J-9以3种不同浓度(0、103、105cfu/mL)按正交实验设计分为9个处理的配比添加到饲料中,进行仿刺参饲养试验。经过30 d养殖后测定仿刺参肠道内蛋白酶、淀粉酶、纤维素酶、氧化物歧化酶和溶菌酶的活性。结果表明:添加益生菌剂可以提高仿刺参消化酶的活性,尤其是蛋白酶和淀粉酶的活性,并且对提高动物免疫功能有积极作用,其中处理6组的蛋白酶活力为对照组的5倍,淀粉酶活力是对照组的两倍,各处理间的纤维素酶活力变化不明显;处理6组的超氧化物歧化酶和溶菌酶活性在所有处理中最高;统计分析表明,处理6组为最佳配比,即乳酸菌L-2添加量为103cfu/mL,芽孢杆菌K-3添加量为105cfu/mL,芽孢杆菌J-9添加量为0。更多还原

【Abstract】 The lactic acid bacteria L-2,Bacillus K-3 and Bacillus J-9 isolated from intestines of sea cucumber Apostichopus japonicus were added to the diets at three rates of 0,103,and 105 cfu/mL and fed to the sea cucumber by orthogonal design for 30 days.The activities of protease,amylase,cellulolytic enzymes,superoxide dismutase and lysozyme were determined in the intestines.The results showed that supplementation of the probiotics improved digestive enzyme activities,especially the protease and amylase in the sea cucumber,and immunity.The protease activity was as high five times in Group 6 as in the control.There was 100% higher amylase activity in Group 6 than that in the control.There were no significant differences in the cellulolytic enzyme activity in all treatments.The maximal superoxide dismutase and lysozyme activities were observed in the group 6(lactic acid bacteria L-2 103 cfu/mL;bacillus K-3 105 cfu/mL;bacillus J-9 0 cfu/mL).更多还原