【摘要】 研究了饲料中维生素C(VC)的含量对花Hemibarbus maculates鱼种生长和免疫指标的影响。在水温为(25.0±3.0)℃的条件下,将14.84 g的花鱼种放养在12个网箱(60 cm×60 cm×120 cm)中,网箱放在室外2个水泥池(6.0 m×2.0 m×1.2 m)中。试验设F0(对照)、F1、F2、F3 4组,分别投喂按0、1 000、2 000和3 000 mg/kg(饲料)剂量添加含量为95%的包膜VC的试验饲料(粗蛋白37.0%、粗脂肪11.0%)。125 d的饲养结果表明:花的成活率为93.33%~98.67%,各组间差异不显著(P>0.05);随着饲料中VC含量的增加,花鱼种的特殊增重率先上升后降低,摄食含973.00 mg/kg VC的饲料时(F1组),鱼的特殊增重率显著高于其它组(P<0.05),饲料系数极显著低于其它组(P<0.01),蛋白质利用率极显著高于其它组(P<0.01);根据二次曲线拟合计算得出,花特殊增重率最高和饲料系数最低时,饲料中VC的含量为1 415.78~1 451.74 mg/kg;花鱼种血清中γ球蛋白含量随着饲料中VC含量的增加先升高再降低,VC含量为973.00 mg/kg时达最高值,该组极显著地高于其它组(P<0.01);花鱼种血清中溶菌酶和超氧化物歧化酶活性随饲料中VC含量的增加而极显著升高(P<0.01),分别在VC含量为2 857.00、1 882.00 mg/kg(F3、F2组)时达最大值。更多还原

【Abstract】 A feeding trial was conducted to evaluate effects of dietary ascorbic acid(vitamin C) on growth and some immune indexes in the juvenile Hemibarbus maculates.The juveniles with 14.84 g in initial body weight were stocked into 12 net cages of each 60 cm ×60 cm×120 cm placed in two flowing water concrete tanks of each 6.0 m×2.0 m×1.2 m at a rate of 25 individuals per cage and fed semi-purified formulated feed containing 37.0% of crude protein and 11.0% of crude lipid,and supplemented with four levels(0,1 000,2 000,3 000 mg/kg diet) of 95% coated-ascorbic acid with triplication for each diet at a water temperature of(25.0±3.0)℃ for 125 days.The survival of the juveniles ranged from 93.33% to 98.67% during the experiment,without significant differences among the groups(P>0.05).The specific growth rate(SGR) of the fish increased with increase in dietary vitamin C levels first,and then decreased.The fish fed the diets containing the vitamin C levels of 1 415.78 mg/kg and 1 451.74 mg/kg had the maximal SGR and the minimal feed conversion ratio(FCR).The maximal serurm γ-globulin level,and activities of lysozyme and superoxide dismutase(SOD),very significantly different from the control group(P<0.01),were found in the fish fed the diets containing the vitamin C levels of 973.00,2 857.00 and 1 882.00 mg/kg,respectively.There were very significantly higher activities of protease,amylase and lipase in the hepatopancreas of Hemibarbus maculates fed the diets supplemented with vitamin C than those in the fish fed the diets without vitamin C supplementation(P<0.01).更多还原