【摘要】 目的:建立准确、灵敏的液相色谱—串联质谱法（LC-MS/MS）测定人血浆中烯丙雌醇的浓度,并评价中国健康女性受试者单剂量口服烯丙雌醇片后的药动学特征。方法:20例女性健康受试者单剂量口服20mg烯丙雌醇片后按时采集血样,以米非司酮为内标,采用APCI正离子选择性反应监测测定烯丙雌醇血浆浓度,计算药动学参数。结果:单剂量口服20mg烯丙雌醇后,烯丙雌醇的主要药动学参数Cmax为（12.48±4.18）ng·ml^-1,Tmax为（1.9±0.8）h,t1/2为（8.4±3.2）h,MRT0-∞为（6.7±2.6）h,AUC0-36为（41.29±14.83）h·ng·ml^-1,AUC0∞为（42.72±15.33）h·ng·ml^-1。结论:建立的LC-MS/MS测定法结果准确可靠,灵敏度高。更多还原

【Abstract】 Objective:To establish an accurate and sensitive LC-MS/MS method for the determination of allylestrenol in plasma end to evaluate its pharmacokinetic study in healthy Chinese women volunteers after po the allylestrenol tablets.Methods:The 20 healthy women volunteers received allylestrenol tablet respectively for a single oral dose of allylestreno120mg.The plasma concentrations of allylestrenol were determined by a validated LC-APCI-MS/MS method with positive ion SRM mode using mifepristone as internal standard.The pharmacokinetic parameters were calculated.Results:The main pharmacokinetic parameters after the single oral dose of allylestrenol tablet（20mg）were as follows:Cmax was（12.48±4.18）ng·ml^-1,Tmax was（1.9±0.8）h,t1/2 was（8.4±3.2）h,MRT0-oo was（6.7±2.6）h,AUC0-36 was（41.29±14.83）h·ng·ml^-1 ,AUC0-∞was（42.72±15.33）h.ng.ml^-1.Concluslons:The validated LC-MS/MS for the determination of allylestrenol in human plasma is accurate,sensitive and reliable.更多还原