【摘要】 利用原子力显微镜对细胞色素C在汞表面生长过程进行研究,发现细胞色素C在汞表面首先形成的是高度稍大于1 nm的吸附单分子层,随着生长时间的增加,吸附在汞表面的细胞色素C分子和溶液中的分子间相互作用,自组装形成生长活性点,生长速度明显加快,生长时间达到8 min时,细胞色素C能形成底部直径达527 nm,高度达138nm,分布密度约为1.2～2.3个/μm~2的细胞色素C分子集聚体.生长机理是吸附自组装机理.细胞色素C的浓度较大地影响其在Hg表面的自组装平衡,随着浓度的减少,细胞色素C所能形成的最大分子集聚体的高度和数目都随之减少,浓度与平均表观高度是非线性的关系.更多还原

【Abstract】 The growth process of cytochrome at the mercury surface was studied with AFM.The single molecule of CC which was a little more than 1 nm high was absorbed at mercury surface at the first minute.The absorbing speed was accelerated when the molecules of CC were interacted at the mercury surface,and then they were further self-assembled to form active bodies.At the eighth minute,the aggregated bodies of CC were formed with the distribution of 1.2～2.3 perμm~2 which were about 138nm in height and about 527nm in diameter.The content of CC solution had great effect on the self-assembling balance.With the decrease of content,the number and height of self-assembling bodies was reduced.The relationship between the content and average height was nonlinearized.更多还原