【摘要】 目的:观察早期肾透明细胞癌及癌旁0.5、1.0、2.0cm组织上皮膜抗原(epithelial membrane antigen,EMA)、肾细胞癌标志物(renal cell carcinoma marker,RCC-Ma)的表达差异,初步探讨早期肾癌行肾部分切除术时的安全切除边距。方法:构建包含44例早期肾透明细胞癌及癌旁0.5、1.0、2.0cm肾皮质的组织芯片,采用免疫组化染色分别观察癌及癌旁(距肿瘤边缘0.5、1.0、2.0cm)组织EMA、RCC-Ma的表达差异。结果:EMA、RCC-Ma在癌及癌旁组织均为细胞膜和(或)胞质染色。癌组织EMA和RCC-Ma强阳性表达率明显高于癌旁组织各组(15.91%/18.18%vs84.09%/79.55%、86.36%/77.27%、79.55%/75.00%,P<0.01);而癌旁组织各组(距肿瘤边缘0.5、1.0、2.0cm)间差异无统计学意义(P>0.05)。结论:0.5cm可能是早期肾透明细胞癌腹腔镜肾部分切除术安全的切除边距。更多还原

【Abstract】 Objective:To observe the differential expression of epithelial membrane antigen(EMA)and renal cell carcinoma marker(RCC-Ma)in early renal carcinoma and corresponding adjacent(0.5,1.0,and 2.0 cm)tissues,so as to estimate the safe margin of laparoscopic partial nephrectomy for renal tumors.Methods:Tissue microarrys,including 44 tissues of clear cell renal cell carcinoma[A] and normal renal cortex tissues of different distances to the tumor:0.5 cm[B],1.0 cm[C] and 2.0 cm[D],were constructed.The expression of EMA and RCC-Ma was examined by immunohistochemical staining in the four groups.Results:Intensive membranous and(or)cytoplasmic staining of EMA and RCC-Ma was observed in the cancer and adjacent tissues.The positive rate in group A was significantly higher than those of the other three groups(A [15.91%/18.18%],B[84.09%/79.55%],C[86.36%/77.27%] and D[79.55%/75.00%],P<0.01).The positive rates of EMA and RCC-Ma expression were not significantly different between the group B,C,and D(P>0.05).Conclusion:Our data denote that,when laparoscopic partial nephrectomy is done for early renal carcinoma,at least 5 mm of normal parenchyma tissue beyond the pesudocapsule should be excised with the tumor.更多还原