【摘要】 建立了高效液相色谱法(HPLC)测定澳洲茄胺盐酸盐含量的方法。色谱柱为Zorbax Extend-C18(250 mm×4.5 mm,5μm),以乙腈-甲醇-乙酸铵为流动相(体积比为60∶35∶5),UV检测器(204 nm),在室温下测定澳洲茄胺盐酸盐含量。澳洲茄胺盐酸盐的质量浓度范围在0.8～160 mg.L-1时(n≥5 000),检测信号与质量浓度线性关系良好Y=166X-1.72×10-14(r=1.000);样品平均回收率(n=9)分别为98.6%。此法简单、重复性好,可用于澳洲茄胺盐酸盐制剂的含量测定。更多还原

【Abstract】 An assay method for the determination of solasodine hydrochloride was given by high performance liquid chromatography(HPLP).The concrete conditions were as follows: Zorbax Extend-C18 column(250 mm×4.5 mm) 5 μm,the volume ratio of CH3OH∶CH3CN∶NH4Ac(mobile phase) 60∶35∶5,flow rate 1.0 mL·min-1,detection wavelength 204 nm,and at room temperature.The linear range of solasodine hydrochloride was from 0.8 to 160 g·L-1(n≥5 000),and there was a good linear correlation between detection signal and mass concentration.The average recovery efficiency(n=9) was 98.6 percent.Result proves that the method is convenient and reliable,and it can be used for the content determination of the aerial parts of solasodine hydrochloride.更多还原