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甜菜氮素同化与蔗糖代谢的关键酶及相关性研究

Activities and correlation study on the key enzymes of nitrogen assimilation and sucrose metabolism in sugar beet roots

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【作者】 王晔赫磊石晓艳李丹马凤鸣

【Author】 WANG Ye, HE Lei, SHI Xiaoyan, LI Dan, MA Fengming (College of Agriculture, Northeast Agricultural University, Harbin 150030, China)

【机构】 东北农业大学农学院

【摘要】 试验以甜菜高糖品种甜研7号为试材,采用框栽方法,进行各生育时期氮素同化酶与蔗糖代谢关键酶的测定。研究表明,在甜菜幼苗期和叶丛形成期,叶片硝酸还原酶(NR)活性分别与根中蔗糖磷酸合成酶(SPS)活性和蔗糖合成酶(SS)分解方向活性呈显著和极显著关系,叶片NR活性与根中SS合成方向活性关系虽呈正相关但未达到显著水平;在甜菜块根增长初期,根中谷氨酰胺合成酶(GS)活性与根中SPS活性呈正相关但未达显著水平、分别与根中SS合成与分解方向活性呈显著和极显著关系。可见根中氮素同化和蔗糖代谢存在既相互联系又相互制约的关系。

【Abstract】 In this dissertation, Tianyan 7 of NZ sugar beet was selected as experimental sample, and experi- ments were made by pool culture. At different growth and development stages there were different correlations between the key enzymes of nitrogen assimilation and sucrose metabolism of sugar beet. The relationships between NR activity and activities of SPS, the decomposition activities of SS in roots were significantly and extremely positive correlations. The relationships between NR activity and the synthesis activities of SS were significantly but not correlations; GS activity in sugar beet roots and the activity of SPS were significantly but not correlations; but activities of GS between synthesis and decomposition activities of SS were significantly and extremely positive correlations. So the relationship of the key enzymes of nitrogen assimilation and sucrose metabolism in sugar beet roots was very important.

【关键词】 甜菜硝酸还原酶谷氨酰胺合成酶蔗糖磷酸合成酶蔗糖合成酶
【Key words】 sugar beetNRGSSPSSS
【基金】 国家自然科学基金项目(30671229);教育部博士学科点专项科研基金(20060224009)
  • 【文献出处】 东北农业大学学报 ,Journal of Northeast Agricultural University , 编辑部邮箱 ,2009年06期
  • 【分类号】S566.3
  • 【被引频次】12
  • 【下载频次】308
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