【摘要】 本研究建立了霸王原生质体再生植株的试验体系。以子叶切块诱导的松软愈伤组织为材料,通过酶法分离出大量有活力的原生质体,原生质体经培养持续分裂形成了愈伤组织,并分化出再生苗。比较了不同培养基和培养密度对原生质体分裂和再生的影响。结果表明,原生质体以2×105个/mL的植板密度,采用液体浅层法培养在附加1.0 mg/L 2,4-二氯苯氧乙酸(2,4-D)、0.2 mg/L 6-苄氨基嘌呤(6-BA)、0.2 mg/L激动素(KT)、500 mg/L水解酪蛋白(CH)、2%蔗糖和0.5 mol/L甘露醇的DPD培养基中,可获得最佳效果,其细胞分裂频率达28.4%左右。转移到附加1.0 mg/L 6-BA、0.5 mg/L萘乙酸(NAA)的MS分化培养基上,获得大量的再生苗。更多还原

【Abstract】 An efficient protocol for plant regeneration from protoplasts of Zygophyllum xanthoxylum was established.The friable calli induced from segments of cotyledon were used for protoplast isolation through enzyme digestion.The effects of different media and plating densities on protoplast divisions and plant regeneration were studied.Sustained cell divisions and colony formation from the protoplasts of Z.xanthoxylum were obtained by a DPD medium containing 1.0 mg/L 2,4-dichlorophenoxyacetic acid(2,4-D),0.2 mg/L 6-benzylaminopurine(6-BA),0.2 mg/L kinetin(KT),0.5 mol/L mannitol,500 mg/L casein hydrolysate,and 2%(W/V) sucrose at a plating density of 2×105 /mL.The division frequency was 28.4%.Regenerated plantlets were obtained when these calli were transferred to MS medium containing 1.0 mg/L 6-BA and 0.5 mg/L(NAA).更多还原