【摘要】 探讨茶黄素对糖尿病肾病大鼠肾小球系膜细胞p38丝裂原活化蛋白激酶(p38MAPK)及细胞外基质(ECM)合成的影响。分别以正常糖(NG)、高糖(HG)、糖基化终产物(AGE)及过氧化氢(H2O2)孵育大鼠肾小球系膜细胞,Western Blot检测系膜细胞中p38MAPK和转化生长因子-β(TGF-β)蛋白表达,酶联免疫吸附实验(ELISA)检测细胞培养液中细胞外基质成分纤维连接蛋白(FN)、Ⅳ型胶原(Col Ⅳ)及层黏连蛋白(LN)的含量。结果显示HG、AGE和H2O2能明显诱导p38MAPK磷酸化和TGF-β蛋白表达增加而导致细胞外基质的积聚,茶黄素能明显抑制p38MAPK的磷酸化活性和TGF-β蛋白表达及减少细胞外基质的积聚。提示茶黄素可通过调节p38MAPK信号转导通路而减少细胞外基质的合成,延缓糖尿病肾小球肥大和肾小球硬化。更多还原

【Abstract】 To study the effect of theaflavin on rat mesangial cells(GMCs) were separately incubated with normal glucose,high glucose,advanced glycosylation end products(AGE) and H2O2.The protein expression of p-p38MAPK and TGF-β was examined by Western blot,and the levels of fibronectin(FN),collagen Ⅳ(ColⅣ),laminine(LN) in the supernatant of cultured GMCs were detected by ELISA.Compared to the control group,high glucose,AGE and H2O2 significantly activated p-p38MAPK and increased the protein expression of TGF-β and extracellar matrix production.The protein expression of p-p38MAPK and TGF-β was inhibited by theaflavin.Compared to GMCs incubated with corresponding stimulators,FN,ColⅣ and LN were expressed at a lower level after pre-treatment of theaflavin.It can be concluded that theaflavin may effectively attenuate diabetic nephropathy progression through down-regulating the expression of TGF-β and extracellar matrix production via p38MAPK.更多还原