【摘要】 目的探讨高迁移率蛋白B1(HMGB1)促进白细胞介素(IL)-2转录表达免疫效应的分子机制。方法构建HMGB1和活化T细胞核因子2(NFAT2)的真核表达质粒,分别单独转染以及共同转染人胚胎肾细胞系293T、人子宫颈癌细胞系Hela,同时转染IL-2报告基因,检测IL-2报告基因的表达活性。观察当HMGB1与NFAT2质粒共同转染细胞时,是否能协同促进IL-2报告基因的活性。结果在293T细胞中,HMGB1或NFAT2单独转染可提高活性倍数相加为6.8倍,而二者共转时报告基因活性与未刺激对照组相比提高了18.4倍。在Hela细胞中,二者单独转染可提高活性倍数相加为49.9倍,而二者共转时报告基因活性与未刺激对照组相比提高了117.7倍。结论HMGB1可与NFAT2可协同促进IL-2报告基因转录表达。更多还原

【Abstract】 Objective To investigate the molecular mechanism of interleukin(IL)-2 production facilitated by high mobility group box-1 protein(HMGB1).Methods Eukaryotic expression plasmid HMGB1 and nuclear factor of activated T cells-2(NFAT2) were transfected into 293T or Hela cells respectively or simultaneously.Reporter gene activity of IL-2 was detected and compared.Coordinate regulation between HMGB1 and NFAT2 was examined.Results Reporter gene activity of IL-2 increased by 6.8 times induced by solo-transfection of HMGB1 or NFAT2 in 293T cells,while increased by 18.4 times induced by co-transfection of HMGB1 and NFAT2 in 293T cells.On the other hand,reporter gene activity increased by 49.9 times induced by solo-transfection of HMGB1 or NFAT2 in Hela cells,while increased by 117.7 times by co-transfection of HMGB1 and NFAT2.Conclusion HMGB1 acts as a coactivator of NFAT2 in promoting IL-2 transcription.更多还原