【摘要】 采用高效液相、碘显色法和苯酚-浓硫酸显色法,对光慈姑的秋水仙碱、淀粉和多糖的含量进行了分析测定;并对多糖提取条件进行了初步探讨。实验结果表明,在562nm波长下,线性方程为:y=0.00807x+0.00713,r=0.99945(n=10);平均回收率=99.36%,淀粉含量0.2753g/g。在489nm波长下,线性方程为y=0.01048x-0.02186,r=0.99905(n=9),平均回收率为98.67%,多糖含量0.1134g/g。在350nm波长下,线性方程为y=8959.47951x-20001.65556,r=0.99737(n=9),平均回收率为98.12%,秋水仙碱含量0.0754 g/g。采用95%乙醇进行沉淀,多糖的含量较高。更多还原

【Abstract】 By use of the absorption of starch-iodine reaction,the absorption of phenol-sulfuric and the high performance liquid chromatography(HPLC),the contents of the starch,polyhexose and colchicine of The Tulipa edulis(Miq.) Bark were measured.the experiments showed at the wavelength of 562nm,the calibration curve was :y=0.00807x+0.00713,r=0.99945(n=10),and the average recovery of starch was 99.36% and the contents was 0.2753g/g.at the wavelength of 489 nm,the calibration curve was : y=0.01048x-0.02186,r=0.99905(n=9) and the average recovery of polyhexose was 98.67% and the contents was 0.1134g/g.at the wavelength of 350nm,the calibration curve wasy=8959.47951x-20001.65556,r=0.99737(n=9) and the contents was 0.0754g/g.The polyhexose was precipitated by the 95% of alcohol,the contents of polyhexose were high.更多还原