【摘要】 目的在脓毒症急性肺损伤(ALI)大鼠模型中观察鞭毛蛋白在其体内可能的致炎症肺损伤作用,及其抗血清可能的对抗炎症损伤的作用。方法复制脓毒症大鼠模型(致伤组),用鞭毛蛋白抗血清对抗(治疗组),并设定对照组(除不结扎、不刺破、不切除盲肠外,其余处理同致伤组),于2、4、6、12、24、48h时,对比观察其动脉血氧分压(PaO2)、湿/干比值(W/D)、血清和肺泡灌洗液(BALF)中鞭毛蛋白和TNF-α含量的变化以及肺组织病理改变。结果与对照组比较:在12、24、48h时,致伤组PaO2显著降低,而W/D显著升高,差异有统计学意义(P<0.01);在6、12、24、48h时,致伤组鞭毛蛋白含量显著升高;在24、48h时,治疗组PaO2显著降低,差异有统计学意义(P<0.01),而W/D显著升高(P<0.01)。在12、24、48h时,致伤组TNF-α含量显著升高,差异有统计学意义(P<0.01);在24、48h时,治疗组TNF-α含量显著升高,差异有统计学意义(P<0.01)。致伤组与治疗组比较:在12、24h时,致伤组PaO2显著低于治疗组,差异有统计学意义(P<0.01),而W/D和TNF-α含量显著高于治疗组,差异有统计学意义(P<0.01)。镜下观察大鼠肺组织发现致伤组在12h后发生显著的病理变化,而治疗组在24h后才发生显著的病理变化。结论鞭毛蛋白是脓毒症时导致大鼠肺炎症损伤的重要致伤因素。更多还原

【Abstract】 Objective To observe the possible lung inflammation injury role of flagellin in acute lung injury(ALI) of rat induced by sepsis and possible anti-inflammation role of anti-flagellin antibody serum.Methods To observe PaO2,Wet/Dry value,flagellin and TNF-α concentration in serum and in bronchus alveoli lavage liquid and pathological changes of lung in injury group,treatment group and control group at 2,4,6,12,24h and 48h.Results PaO2 was significantly lower,W/D was significantly higher in injury group at 12,24,48h;flagellin concentration was significantly higher in injury group at 6,12,24,48h,PaO2 was significantly lower and W/D was significantly higher in anti-flagellin antibody serum group only at 24,48h than in control group(P<0.01);TNF-α concentration was significantly higher in injury group at 12,24 and 48h,and was significantly higher in flagellin antibody serum group only at 24h,48h than in control group(P<0.01).Contrast between injury group and anti-flagellin antibody serum group:PaO2 was significantly lower in injury group,but W/D and TNF-αconcentration were significantly higher in injury group at 12,24h(P<0.01).There were some obvious pathological changes of lung in injury group after 12h and in anti-flagellin antibody serum group after 24h.Conclusion Flagellin is important relative factor in ALI of rat induced by sepsis.更多还原