【摘要】 目的探讨牙龈组织中IL-8与慢性牙周炎的关系。方法采用双抗夹心酶联免疫吸附法(ELISA)测定20例慢性牙周炎患者与20例正常对照者龈沟液(GCF)及血清中的IL-8含量,并用免疫组化和原位杂交法,分析IL-8的检出率及IL-8分泌细胞在牙龈组织中的分布。结果牙周炎患者GCF中IL-8含量明显高于对照组GCF中的含量(P<0.01)及牙周炎患者血清中的含量(P<0.01);慢性牙周炎患者中的IL-8的检出率均高于正常对照者,IL-8阳性细胞分布于牙龈的上皮和结缔组织中。结论IL-8主要来源于牙龈组织局部,IL-8参与了牙周炎的病理过程。更多还原

【Abstract】 Objective To study the relation of interleukin-8(IL-8) in periodontitis.Methods IL-8 contents in gingival crevicular fluid(GCF) were measured in periodontitis and healthy controls with sandwich enzyme-linked immunosorbent assay(ELISA).In addition,IL-8 contents in serum were studied in periodontitis and healthy controls at the same time.And by immunohistochemical and in situ hybridization analysis of IL-8 detection rate and IL-8 secretion of cells in the gingival tissue distribution.Results IL-8 level in GCF was markedly higher in periodontitis than in healthy controls(P<0.001) and than in serum of periodontitis(P<0.001).Patients with chronic periodontitis in IL-8 detection rate were higher than normal controls,IL-8-positive cells distributed in the gingival epithelium and connective tissue.Conclusion IL-8 mainly come from the gingival tissue,IL-8 involved in the pathological process of periodontitis.更多还原