【摘要】 报道了水解酶催化二乙基锌与芳香醛的加成反应,对反应条件进行了优化.在最适条件(嗜热酯酶APE1547为酶源,反应温度40oC,氯仿为溶剂,4-Cl-苯甲醛为底物)下,加成反应生成的光学活性醇产率最高达78%,ee值最高可达56%.通过实验结果和分子动力学分析对可能的反应机理进行了推测.本研究进一步拓展了酶的非专一性.更多还原

【Abstract】 A novel application of hydrolase as catalyst for the addition of diethylzinc to aromatic aldehydes is reported.Optimum conditions were screened out.Under the optimum conditions(i.e.,reaction at 40 oC using hyperthermophilice esterase APE1547,4-Cl-benzaldehyde substrate,and trichloromethane solvent),this reaction provided optically active secondary alcohol with an enantiomeric excess up to 56% and a yield up to 78%.The possible mechanism was proposed based on experimental observations and molecular dynamics simulation.These observations extend the phenomenon of enzyme promiscuity.更多还原