【摘要】 试验结果表明,仿刺参酸性磷酸酶的最佳提取缓冲液为pH 7.5的Tris-HCl缓冲液,最佳沉淀条件为饱和度为80%的硫酸铵。仿刺参体壁和肠道中的酸性磷酸酶最适反应条件均为:40℃,pH4.0。Ca2+、Mg2+和Mn2+对体壁酸性磷酸酶有激活作用,而Fe3+、Fe2+、Zn2+、Cu2+、Ba2+对其有抑制作用,其中Cu2+的抑制作用最强;肠道酸性磷酸酶表现的特征与之类似,但Zn2+的抑制作用最强。PAGE电泳结果表明,仿刺参体壁和肠道中的酸性磷酸酶类型不同,肠道中有3种同工酶。更多还原

【Abstract】 Extraction and properties of acid phosphatase were investigated from sea cucumber(Apostichopus japonicus).Tris-HCl buffer(pH 7.5)was found to be the optimal extraction solution and 80% of saturated(NH4)2SO4 for optimum precipitation.The optimal temperature and pH of acid phosphatase were 40 ℃,and 4.0 in body wall and gut.The crude acid phosphatase from body wall was activated by Ca2+,Mg2+and Mn2+,and inhibited by Fe3+,Fe2+,Zn2+,Cu2+ and Ba2+,in which Cu2+ was the strongest inhibitor.The crude acid phosphatase from the gut had nearly the same property as that from the body walls,and Zn2+ showed the strongest inhibition.The PAGE revealed that the acid phosphatases in the body walls were different from those in the guts,in which there were three isoenzymes of acid phosphatases.更多还原